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PXD016070

PXD016070 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAn extended PAXT connection is required for turnover of nuclear polyadenylated RNA in human cells
DescriptionRecruitment of the human ribonucleolytic RNA exosome to nuclear polyadenylated (pA+) RNA is facilitated by the Poly(A) Tail eXosome Targeting (PAXT) connection. Besides its core dimer, formed by the exosome co-factor MTR4 and the ZFC3H1 protein, the PAXT connection remains poorly defined. By characterizing nuclear pA+-RNA bound proteomes as well as MTR4-ZFC3H1 containing complexes in conditions favoring PAXT assembly, we here uncover three additional proteins required for PAXT function: ZC3H3, RBM26/RBM27 and the known PAXT-associated protein, PABPN1. The zinc-finger protein ZC3H3 interacts directly with MTR4-ZFC3H1 and loss of either identified PAXT component results in the accumulation of PAXT substrates. Collectively, our results establish new factors involved in the turnover of nuclear pA+ RNA and suggest that these are limiting for PAXT activity.
HostingRepositoryjPOST
AnnounceDate2019-10-31
AnnouncementXMLSubmission_2022-09-18_02:38:33.582.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterKoshi Imami
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListS-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-10-29 17:52:18ID requested
12019-10-30 16:56:47announced
22020-05-17 17:20:43announced2020-05-18: Updated PubMed.
32021-02-04 16:50:14announced2021-02-04: Updated FTP location.
42022-09-18 02:38:34announced2022-09-18: Updated FTP location.
Publication List
Silla T, Schmid M, Dou Y, Garland W, Milek M, Imami K, Johnsen D, Polak P, Andersen JS, Selbach M, Landthaler M, Jensen TH, The human ZC3H3 and RBM26/27 proteins are critical for PAXT-mediated nuclear RNA decay. Nucleic Acids Res, 48(5):2518-2530(2020) [pubmed]
Keyword List
submitter keyword: nuclear RNA exosome, RNA degradation, the PAXT connection, ZC3H3, RBM26, RBM27, PABPN1
Contact List
Torben Heick Jensen
lab head
Koshi Imami
contact affiliationKyoto University
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000693/