PXD016070

PXD016070 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	An extended PAXT connection is required for turnover of nuclear polyadenylated RNA in human cells
Description	Recruitment of the human ribonucleolytic RNA exosome to nuclear polyadenylated (pA+) RNA is facilitated by the Poly(A) Tail eXosome Targeting (PAXT) connection. Besides its core dimer, formed by the exosome co-factor MTR4 and the ZFC3H1 protein, the PAXT connection remains poorly defined. By characterizing nuclear pA+-RNA bound proteomes as well as MTR4-ZFC3H1 containing complexes in conditions favoring PAXT assembly, we here uncover three additional proteins required for PAXT function: ZC3H3, RBM26/RBM27 and the known PAXT-associated protein, PABPN1. The zinc-finger protein ZC3H3 interacts directly with MTR4-ZFC3H1 and loss of either identified PAXT component results in the accumulation of PAXT substrates. Collectively, our results establish new factors involved in the turnover of nuclear pA+ RNA and suggest that these are limiting for PAXT activity.
HostingRepository	jPOST
AnnounceDate	2019-10-31
AnnouncementXML	Submission_2022-09-18_02:38:33.582.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Koshi Imami
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-29 17:52:18	ID requested
1	2019-10-30 16:56:47	announced
2	2020-05-17 17:20:43	announced	2020-05-18: Updated PubMed.
3	2021-02-04 16:50:14	announced	2021-02-04: Updated FTP location.
⏵ 4	2022-09-18 02:38:34	announced	2022-09-18: Updated FTP location.

Publication List

Silla T, Schmid M, Dou Y, Garland W, Milek M, Imami K, Johnsen D, Polak P, Andersen JS, Selbach M, Landthaler M, Jensen TH, The human ZC3H3 and RBM26/27 proteins are critical for PAXT-mediated nuclear RNA decay. Nucleic Acids Res, 48(5):2518-2530(2020) [pubmed]

Silla T, Schmid M, Dou Y, Garland W, Milek M, Imami K, Johnsen D, Polak P, Andersen JS, Selbach M, Landthaler M, Jensen TH, The human ZC3H3 and RBM26/27 proteins are critical for PAXT-mediated nuclear RNA decay. Nucleic Acids Res, 48(5):2518-2530(2020) [pubmed]

Keyword List

submitter keyword: nuclear RNA exosome, RNA degradation, the PAXT connection, ZC3H3, RBM26, RBM27, PABPN1

submitter keyword: nuclear RNA exosome, RNA degradation, the PAXT connection, ZC3H3, RBM26, RBM27, PABPN1

Contact List

Torben Heick Jensen
lab head
Koshi Imami
contact affiliation	Kyoto University
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000693/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000693/