PXD016063

PXD016063 is an original dataset announced via ProteomeXchange.

Title	Unique features of mRNA translation initiation in trypanosomatids
Description	The 43S pre-initiation complex (PIC) assembly requires establishment of numerous interactions among eukaryotic initiation factors (eIFs), Met-tRNAiMet and the small ribosomal subunit (40S). Owing to several differences in the structure and composition of kinetoplastidian 40S compared to their mammalian counterparts, translation initiation in trypanosomatids is suspected to display substantial variability. Here, we determined the structure of the 43S PIC from Trypanosoma cruzi, the Chagas disease parasite, showing numerous specific features, such as different eIF3 structure and interactions with the large rRNA expansion segments 9S, 7S and 6S, and the association of a kinetoplastid-specific ~245 kDa DDX60-like helicase. We also revealed a previously undetermined binding site of the eIF5 C-terminal domain, and terminal tails of eIF2β, eIF1, eIF1A and eIF3 c and d subunits, uncovering molecular details of their critical activities.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:01:18.657.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lauriane Kuhn
SpeciesList	scientific name: Leishmania tarentolae; NCBI TaxID: 5689; scientific name: Trypanosoma cruzi strain CL Brener; NCBI TaxID: 353153;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-29 03:47:37	ID requested
1	2020-03-25 00:40:44	announced
⏵ 2	2024-10-22 05:01:22	announced	2024-10-22: Updated project metadata.

Bochler A, Querido JB, Prilepskaja T, Soufari H, Simonetti A, Del Cistia ML, Kuhn L, Ribeiro AR, Val, á, š, ek LS, Hashem Y, Structural Differences in Translation Initiation between Pathogenic Trypanosomatids and Their Mammalian Hosts. Cell Rep, 33(12):108534(2020) [pubmed]

10.1016/j.celrep.2020.108534;

submitter keyword: translation, trypanosomatids, pre-initiation complex

Yaser Hashem
contact affiliation	INSERM U1212, Institut Européen de Chimie et Biologie, University of Bordeaux, Pessac 33607, France
contact email	yaser.hashem@u-bordeaux.fr
lab head
Lauriane Kuhn
contact affiliation	CNRS, IBMC, FRC1589
contact email	l.kuhn@ibmc-cnrs.unistra.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/03/PXD016063

PRIDE project URI

• PRIDE
  1. PXD016063
     1. Label: PRIDE project
     2. Name: Unique features of mRNA translation initiation in trypanosomatids