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PXD016063

PXD016063 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleUnique features of mRNA translation initiation in trypanosomatids
DescriptionThe 43S pre-initiation complex (PIC) assembly requires establishment of numerous interactions among eukaryotic initiation factors (eIFs), Met-tRNAiMet and the small ribosomal subunit (40S). Owing to several differences in the structure and composition of kinetoplastidian 40S compared to their mammalian counterparts, translation initiation in trypanosomatids is suspected to display substantial variability. Here, we determined the structure of the 43S PIC from Trypanosoma cruzi, the Chagas disease parasite, showing numerous specific features, such as different eIF3 structure and interactions with the large rRNA expansion segments 9S, 7S and 6S, and the association of a kinetoplastid-specific ~245 kDa DDX60-like helicase. We also revealed a previously undetermined binding site of the eIF5 C-terminal domain, and terminal tails of eIF2β, eIF1, eIF1A and eIF3 c and d subunits, uncovering molecular details of their critical activities.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:01:18.657.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLauriane Kuhn
SpeciesList scientific name: Leishmania tarentolae; NCBI TaxID: 5689; scientific name: Trypanosoma cruzi strain CL Brener; NCBI TaxID: 353153;
ModificationListmonohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-10-29 03:47:37ID requested
12020-03-25 00:40:44announced
22024-10-22 05:01:22announced2024-10-22: Updated project metadata.
Publication List
Bochler A, Querido JB, Prilepskaja T, Soufari H, Simonetti A, Del Cistia ML, Kuhn L, Ribeiro AR, Val, á, š, ek LS, Hashem Y, Structural Differences in Translation Initiation between Pathogenic Trypanosomatids and Their Mammalian Hosts. Cell Rep, 33(12):108534(2020) [pubmed]
10.1016/j.celrep.2020.108534;
Keyword List
submitter keyword: translation, trypanosomatids, pre-initiation complex
Contact List
Yaser Hashem
contact affiliationINSERM U1212, Institut Européen de Chimie et Biologie, University of Bordeaux, Pessac 33607, France
contact emailyaser.hashem@u-bordeaux.fr
lab head
Lauriane Kuhn
contact affiliationCNRS, IBMC, FRC1589
contact emaill.kuhn@ibmc-cnrs.unistra.fr
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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