PXD016063 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Unique features of mRNA translation initiation in trypanosomatids |
Description | The 43S pre-initiation complex (PIC) assembly requires establishment of numerous interactions among eukaryotic initiation factors (eIFs), Met-tRNAiMet and the small ribosomal subunit (40S). Owing to several differences in the structure and composition of kinetoplastidian 40S compared to their mammalian counterparts, translation initiation in trypanosomatids is suspected to display substantial variability. Here, we determined the structure of the 43S PIC from Trypanosoma cruzi, the Chagas disease parasite, showing numerous specific features, such as different eIF3 structure and interactions with the large rRNA expansion segments 9S, 7S and 6S, and the association of a kinetoplastid-specific ~245 kDa DDX60-like helicase. We also revealed a previously undetermined binding site of the eIF5 C-terminal domain, and terminal tails of eIF2β, eIF1, eIF1A and eIF3 c and d subunits, uncovering molecular details of their critical activities. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:01:18.657.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Lauriane Kuhn |
SpeciesList | scientific name: Leishmania tarentolae; NCBI TaxID: 5689; scientific name: Trypanosoma cruzi strain CL Brener; NCBI TaxID: 353153; |
ModificationList | monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-10-29 03:47:37 | ID requested | |
1 | 2020-03-25 00:40:44 | announced | |
⏵ 2 | 2024-10-22 05:01:22 | announced | 2024-10-22: Updated project metadata. |
Publication List
Bochler A, Querido JB, Prilepskaja T, Soufari H, Simonetti A, Del Cistia ML, Kuhn L, Ribeiro AR, Val, á, š, ek LS, Hashem Y, Structural Differences in Translation Initiation between Pathogenic Trypanosomatids and Their Mammalian Hosts. Cell Rep, 33(12):108534(2020) [pubmed] |
10.1016/j.celrep.2020.108534; |
Keyword List
submitter keyword: translation, trypanosomatids, pre-initiation complex |
Contact List
Yaser Hashem |
contact affiliation | INSERM U1212, Institut Européen de Chimie et Biologie, University of Bordeaux, Pessac 33607, France |
contact email | yaser.hashem@u-bordeaux.fr |
lab head | |
Lauriane Kuhn |
contact affiliation | CNRS, IBMC, FRC1589 |
contact email | l.kuhn@ibmc-cnrs.unistra.fr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD016063
- Label: PRIDE project
- Name: Unique features of mRNA translation initiation in trypanosomatids