PXD016047

PXD016047 is an original dataset announced via ProteomeXchange.

Title	A comprehensive CHO SWATH-MS spectral library for accurate quantitative profiling of 10k proteins
Description	A comprehensive Chinese hamster ovary (CHO)-cell specific spectral library, containing extensive information of high-quality spectral ions covering more than 10k CHO cell proteins, was constructed to provide confident and reproducible protein identification and quantification in data-independent SWATH-MS analysis. The applicability of CHO spectral library was tested in the analyses of different CHO samples including whole cell lysate, harvested cell culture fluids, and downstream processing samples. The portability of CHO spectral library was also demonstrated in the processing and analyses of SWATH-MS data sets collected from multiple LC-MS instrumental setups and various CHO cell lines.
HostingRepository	PRIDE
AnnounceDate	2020-07-20
AnnouncementXML	Submission_2020-08-16_22:27:18.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kae Hwan Sim
SpeciesList	scientific name: Cricetulus griseus; NCBI TaxID: 10029;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	TripleTOF 5600+; TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-28 03:27:29	ID requested
1	2020-07-19 22:03:17	announced
⏵ 2	2020-08-16 22:27:19	announced	2020-08-17: Updated publication reference for PubMed record(s): 32782267.

Sim KH, Liu LC, Tan HT, Tan K, Ng D, Zhang W, Yang Y, Tate S, Bi X, A comprehensive CHO SWATH-MS spectral library for robust quantitative profiling of 10,000 proteins. Sci Data, 7(1):263(2020) [pubmed]

submitter keyword: Chinese hamster ovary cells, SWATH-MS, LC-MS/MS, TripleTOF 6600

Xuezhi Bi
contact affiliation	Proteomics Laboratory, Bioprocessing Technology Institute, A*STAR, Singapore
contact email	bi_xuezhi@bti.a-star.edu.sg
lab head
Kae Hwan Sim
contact affiliation	Bioprocessing Technology Institute, A*STAR
contact email	sim_kae_hwan@bti.a-star.edu.sg
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/07/PXD016047

PRIDE project URI

• PRIDE
  1. PXD016047
     1. Label: PRIDE project
     2. Name: A comprehensive CHO SWATH-MS spectral library for accurate quantitative profiling of 10k proteins