PXD016043

PXD016043 is an original dataset announced via ProteomeXchange.

Title	Extrinsic inflammatory, but not intrinsic oligodendroglial factors contribute to oligodendroglial differentiation block in multiple sclerosis
Description	Multiple sclerosis (MS) is the most frequent demyelinating disease and despite significant advances in the immunotherapy, disease progression still cannot be prevented. Promotion of remyelination, an endogenous repair process, represents a promising new treatment approach. However, spontaneous remyelination frequently fails in MS lesions due to an impaired differentiation of progenitor cells into mature, myelinating oligodendrocytes. Intrinsic oligodendroglial and extrinsic inflammatory factors may contribute to this differentiation block. Therefore, we compared induced pluripotent stem cell (iPSC)-derived oligodendrocytes (hiOL) from MS patients and healthy controls as well as their response to extrinsic factors. While functional capabilities or proteome compostion of both cell types were virtually indistinguishable, we discovered that Interferon-gamma (IFNγ) producing immune cells significantly impaired oligodendroglial differentiation and observed no differences in the functional capabilities or the proteome. In summary, these data indicate that the oligodendroglial differentiation block is not due to intrinsic oligodendroglial factors, but rather caused by the inflammatory environment present in MS lesions. These findings may contribute to the development of remyelination promoting strategies in MS.
HostingRepository	PRIDE
AnnounceDate	2020-09-04
AnnouncementXML	Submission_2020-09-15_23:09:48.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hannes Drexler
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-28 03:00:12	ID requested
1	2020-09-04 04:19:30	announced
⏵ 2	2020-09-15 23:12:56	announced	2020-09-16: Updated publication reference for PubMed record(s): 32894330.

Starost L, Lindner M, Herold M, Xu YKT, Drexler HCA, He, ß K, Ehrlich M, Ottoboni L, Ruffini F, Stehling M, R, ö, pke A, Thomas C, Sch, ö, ler HR, Antel J, Winkler J, Martino G, Klotz L, Kuhlmann T, Extrinsic immune cell-derived, but not intrinsic oligodendroglial factors contribute to oligodendroglial differentiation block in multiple sclerosis. Acta Neuropathol, 140(5):715-736(2020) [pubmed]

submitter keyword: Induced pluripotent stem cells, oligodendrocytes, proteomics, mass spectrometry, relapsing-remitting multiple sclerosis, remyelination, IFNγ

Hannes C. A. Drexler
contact affiliation	Max Planck Institute for Biomedical Research Bioanalytical Mass Spectrometry Unit Röntgenstr. 20 48149 Münster Germany
contact email	hannes.drexler@mpi-muenster.mpg.de
lab head
Hannes Drexler
contact affiliation	Bioanalytical Mass Spectrometry
contact email	hannes.drexler@mpi-muenster.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD016043
     1. Label: PRIDE project
     2. Name: Extrinsic inflammatory, but not intrinsic oligodendroglial factors contribute to oligodendroglial differentiation block in multiple sclerosis