PXD016042

PXD016042 is an original dataset announced via ProteomeXchange.

Title	Zinc limitation in Klebsiella pnuemoniae
Description	Nutrient adaptation is key in limiting environments for the promotion of microbial growth and survival. In microbial systems, zinc is an important component for many cellular processes and bioavailability varies greatly among different conditions. In the bacterium, Klebsiella pneumoniae, the impact of zinc limitation has not been explored at the protein level. Here, we apply a mass spectrometry-based quantitative proteomics strategy to profile the global impact of zinc limitation on the cellular proteome and extracellular environment (secretome) of K. pneumoniae. Our data defines the impact of zinc on proteins involved in transcriptional regulation and emphasizes the modulation of a vast array of proteins associated with zinc acquisition, transport, and binding. We also identify proteins in the extracellular environment associated with conventional and nonconventional modes of secretion. In particular, we focus on the impact of zinc on an uncharacterized cation transporter and the role of the ChaB transporter in influencing bacterial growth, capsule production, nad virulence. Moreover, we validate our results through transcriptional profiling of genes reprsessed under high abudnance of a histidine repression operon, hutC. Overall, we provide evidence of novel connections between zinc availability and capsule production in a bacterial system and define new roles for a transporter.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:50:46.251.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jennifer Geddes-McAlister
SpeciesList	scientific name: Klebsiella pneumoniae; NCBI TaxID: 573;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-28 02:27:42	ID requested
1	2021-02-12 06:30:43	announced
⏵ 2	2023-11-14 08:50:46	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: virulence, Klebsiella, transport, quantiative proteomics,zinc, proteome, secretome

Jennifer Geddes-McAlister
contact affiliation	University of Guelph, Molecular and Cellular Biology Department, Guelph, ON, N1G 2W1, Canada
contact email	jgeddesm@uoguelph.ca
lab head
Jennifer Geddes-McAlister
contact affiliation	University of Guelph
contact email	jgeddesm@uoguelph.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD016042
     1. Label: PRIDE project
     2. Name: Zinc limitation in Klebsiella pnuemoniae