PXD016039

PXD016039 is an original dataset announced via ProteomeXchange.

Title	Age-associated changes in human CD4+ T cells point to mitochondrial dysfunction consequent to impaired autophagy
Description	To gain understanding on the mechanisms that drive immunosenescence in humans, we examined CD4+ T cells obtained from younger (20-39 years-old) and older (70+ years-old) healthy participants of the Baltimore Longitudinal Study on Aging (BLSA). We found that mitochondrial proteins involved in the electron transport chain were overrepresented in cells from older participants, with prevalent dysregulation of oxidative phosphorylation and energy metabolism molecular pathways. Surprisingly, gene transcripts coding for mitochondrial proteins pertaining to oxidative phosphorylation and electron transport chain pathways were underrepresented in older individuals. Paralleling the observed decrease in gene expression, mitochondrial respiration was impaired in CD4+ T cells from older subjects. Though mitochondrial number in both naïve and memory cells visualized with electron microcopy was similar in older versus younger participants, there were a significantly higher number of autophagosomes, many of them containing undegraded mitochondria, in older individuals. The presence of mitochondria inside the accumulated autophagic compartments in CD4+ T cells from older individuals was confirmed by immunofluorescence. These findings suggest that older age is associated with persistence of dysfunctional mitochondria in CD4+ T lymphocytes caused by defective mitochondrial turnover by autophagy, which may trigger chronic inflammation and contribute to the impairment of immune defense in older persons.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:58:21.903.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Arsun Bektas
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-28 02:27:24	ID requested
1	2019-11-27 02:46:16	announced
⏵ 2	2024-10-22 04:58:22	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: autophagy,proteomics, mitochondria, CD4+ T cells, aging

Luigi Ferrucci
contact affiliation	Translational Gerontology Branch, National Institute on Aging, National Institutes of Health, USA
contact email	ferruccilu@mail.nih.gov
lab head
Arsun Bektas
contact affiliation	NIH/NIA
contact email	bektasar@mail.nih.gov
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD016039

PRIDE project URI

• PRIDE
  1. PXD016039
     1. Label: PRIDE project
     2. Name: Age-associated changes in human CD4+ T cells point to mitochondrial dysfunction consequent to impaired autophagy