PXD016032

PXD016032 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Optimization of data-independent acquisition-MS for deep proteome analysis
Description	Data-independent acquisition (DIA)-MS analysis overtops the existing data-dependent acquisition (DDA)-MS analysis enabling deep proteome coverage and precise relative quantitative analysis in single-shot LC-MS/MS. We constructed a single-shot LC-MS/MS system with an MS measurement time of 90 min for a highly sensitive and deep proteomic analysis by optimizing the parameters of DIA and nanoLC. Furthermore, we applied this system to the proteomic analysis of cerebrums in germ-free and specific pathogen-free mice.
HostingRepository	jPOST
AnnounceDate	2019-10-28
AnnouncementXML	Submission_2022-09-18_02:52:15.372.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yusuke Kawashima
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: cellular organisms; NCBI TaxID: 131567;
ModificationList	S-carboxamidomethyl-L-cysteine; unknown modification
Instrument	Q Exactive HF-X

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-27 15:55:45	ID requested
1	2019-10-27 15:55:50	announced
2	2020-08-21 17:35:16	announced	2020-08-22: Updated PubMed.
3	2021-02-04 17:20:56	announced	2021-02-04: Updated FTP location.
⏵ 4	2022-09-18 02:52:16	announced	2022-09-18: Updated FTP location.

Publication List

Kawashima Y, Watanabe E, Umeyama T, Nakajima D, Hattori M, Honda K, Ohara O, Optimization of Data-Independent Acquisition Mass Spectrometry for Deep and Highly Sensitive Proteomic Analysis. Int J Mol Sci, 20(23):(2019) [pubmed]

Kawashima Y, Watanabe E, Umeyama T, Nakajima D, Hattori M, Honda K, Ohara O, Optimization of Data-Independent Acquisition Mass Spectrometry for Deep and Highly Sensitive Proteomic Analysis. Int J Mol Sci, 20(23):(2019) [pubmed]

Keyword List

submitter keyword: DIA, SWATH, Single shot, HEK293 cell, cerebrum

submitter keyword: DIA, SWATH, Single shot, HEK293 cell, cerebrum

Contact List

Yusuke Kawashima
lab head
Yusuke Kawashima
contact affiliation	Kazusa DNA Research Institute
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000691/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000691/