PXD016023

PXD016023 is an original dataset announced via ProteomeXchange.

Title	Similar but not identical – a quadruple pack of LSU (RESPONSE TO LOW SULFUR) from Arabidopsis thaliana
Description	The members of plant-specific LSU (RESPONSE TO LOW SULFUR) family were first identified as strongly induced during sulfur starvation. Molecular function of these protein remains unknown, however they were identified as important stress-related hubs by several research groups. In Arabidopsis thaliana there are four members of LSU family. These proteins are involved in multiple protein-protein interactions and literature data suggest that they can integrate abiotic and biotic stress responses. LSU proteins are small and have the coiled-coil structure. Additionally to binding with other proteins they can form homo- and heterodimers and possibly also multimers. In this work we investigated interactions between different monomers of LSU1-4 using Y2H and BiFC methods. The differences in strength of various homo- and heterodimers formation were observed. The constructed by us structural models of the LSU1-4 homo- and heterodimers were in agreement with the experimental observations concerning differences in strength of dimers formation and might help understanding interaction of LSU with other partners. Since previously the partners of LSU were identified using the Y2H approach we decided to obtain the lists of LSU interactors in plants using the TAP-tagged LSU1-4. Interaction of LSUs with a few selected proteins from the lists was verified by Y2H and BiFC.
HostingRepository	PRIDE
AnnounceDate	2020-08-12
AnnouncementXML	Submission_2020-08-12_02:51:50.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dominik Cysewski
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-25 02:35:29	ID requested
⏵ 1	2020-08-12 02:51:50	announced

Dataset with its publication pending

submitter keyword: Arabidopsis, sulfur, CoIP,

Agnieszka Sirko
contact affiliation	Institute of Biochemistry and Biophysics, Polish Academy of Sciences Pawinskiego 5a, 02-106 Warsaw POLAN
contact email	asirko@ibb.waw.pl
lab head
Dominik Cysewski
contact affiliation	Institute of Biochemistry and Biophysics Polish Academy of Sciences
contact email	dominikcysewski@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/08/PXD016023

PRIDE project URI

• PRIDE
  1. PXD016023
     1. Label: PRIDE project
     2. Name: Similar but not identical – a quadruple pack of LSU (RESPONSE TO LOW SULFUR) from Arabidopsis thaliana