⮝ Full datasets listing

PXD016022

PXD016022 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDetermining MELK inhibitor specificity using MIB/MS
DescriptionThe key objectives of this study were to evaluate the selectivity profiles of three MELK inhibitors, 8a, HTH, and OTS, using a cell-based assay, in order to identify a highly selective inhibitor to subsequently investigate MELK function. To this end, we utilized a chemical proteomics approach called multiplexed kinase inhibitor beads/mass spectrometry (MIB/MS) to characterize the selectivity of these MELK inhibitors in TNBC cells.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:58:32.997.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLaura Herring
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-10-25 02:35:16ID requested
12020-01-09 01:16:02announced
22024-10-22 04:58:35announced2024-10-22: Updated project metadata.
Publication List
McDonald IM, Grant GD, East MP, Gilbert TSK, Wilkerson EM, Goldfarb D, Beri J, Herring LE, Vaziri C, Cook JG, Emanuele MJ, Graves LM, Mass spectrometry-based selectivity profiling identifies a highly selective inhibitor of the kinase MELK that delays mitotic entry in cancer cells. J Biol Chem, 295(8):2359-2374(2020) [pubmed]
10.1074/jbc.ra119.011083;
Keyword List
submitter keyword: chemoproteomics, LFQ,kinase enrichment
Contact List
Lee M. Graves
contact affiliationPharmacology Department, UNC School of Medicine, UNC-Chapel Hill
contact emaillmg@med.unc.edu
lab head
Laura Herring
contact affiliationUNC-Chapel Hill
contact emaillaura_herring@med.unc.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/01/PXD016022
PRIDE project URI
Repository Record List
[ + ]