PXD016022 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Determining MELK inhibitor specificity using MIB/MS |
Description | The key objectives of this study were to evaluate the selectivity profiles of three MELK inhibitors, 8a, HTH, and OTS, using a cell-based assay, in order to identify a highly selective inhibitor to subsequently investigate MELK function. To this end, we utilized a chemical proteomics approach called multiplexed kinase inhibitor beads/mass spectrometry (MIB/MS) to characterize the selectivity of these MELK inhibitors in TNBC cells. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:58:32.997.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Laura Herring |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-10-25 02:35:16 | ID requested | |
1 | 2020-01-09 01:16:02 | announced | |
⏵ 2 | 2024-10-22 04:58:35 | announced | 2024-10-22: Updated project metadata. |
Publication List
McDonald IM, Grant GD, East MP, Gilbert TSK, Wilkerson EM, Goldfarb D, Beri J, Herring LE, Vaziri C, Cook JG, Emanuele MJ, Graves LM, Mass spectrometry-based selectivity profiling identifies a highly selective inhibitor of the kinase MELK that delays mitotic entry in cancer cells. J Biol Chem, 295(8):2359-2374(2020) [pubmed] |
10.1074/jbc.ra119.011083; |
Keyword List
submitter keyword: chemoproteomics, LFQ,kinase enrichment |
Contact List
Lee M. Graves |
contact affiliation | Pharmacology Department, UNC School of Medicine, UNC-Chapel Hill |
contact email | lmg@med.unc.edu |
lab head | |
Laura Herring |
contact affiliation | UNC-Chapel Hill |
contact email | laura_herring@med.unc.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/01/PXD016022 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD016022
- Label: PRIDE project
- Name: Determining MELK inhibitor specificity using MIB/MS