PXD016021

PXD016021 is an original dataset announced via ProteomeXchange.

Title	Quantitative Proteomics Analysis to Determine Specificity of PRC2 Degrader
Description	To assess the effects of UNC6852 treatment on cellular protein levels more broadly, we performed global proteomics experiments using tandem mass tag (TMT) quantification comparing HeLa cells treated with UN6852 (10 μM, 24 hrs) to DMSO treated control cells. Whole proteome analysis resulted in the identification of >60,000 peptides corresponding to 5,452 quantifiable proteins. Notably, these data revealed that EED and EZH2 were selectively degraded by UNC6852 within the proteome.
HostingRepository	PRIDE
AnnounceDate	2020-01-09
AnnouncementXML	Submission_2020-01-09_01:40:33.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Laura Herring
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-25 02:35:13	ID requested
⏵ 1	2020-01-09 01:56:12	announced

Potjewyd F, Turner AW, Beri J, Rectenwald JM, Norris-Drouin JL, Cholensky SH, Margolis DM, Pearce KH, Herring LE, James LI, Degradation of Polycomb Repressive Complex 2 with an EED-Targeted Bivalent Chemical Degrader. Cell Chem Biol, 27(1):47-56.e15(2020) [pubmed]

submitter keyword: TMT, chemoproteomics

Lindsey James
contact affiliation	Center for Integrative Chemical Biology and Drug Discovery, UNC Eshelman School of Pharmacy, UNC-Chapel Hill
contact email	ingerman@email.unc.edu
lab head
Laura Herring
contact affiliation	UNC-Chapel Hill
contact email	laura_herring@med.unc.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/01/PXD016021

PRIDE project URI

• PRIDE
  1. PXD016021
     1. Label: PRIDE project
     2. Name: Quantitative Proteomics Analysis to Determine Specificity of PRC2 Degrader