PXD016011

PXD016011 is an original dataset announced via ProteomeXchange.

Title	Pronucleotide probe for in vivo imaging of protein AMPylation.
Description	Protein AMPylation is a prevalent posttranslational modification in human cells involved in the regulation of unfolded protein response and neural development. Here we present a novel pro-N6azA probe suitable for in vivo imaging and chemical proteomics profilling of AMPylated proteins. The pro-N6azA probe provides stable fluorescent labelling in living cells accesible by straightforward strain-promoted azide-alkyne click reaction. Application of this probe may contribute significantly to the analysis of protein AMPylation during various metabolic processes including neurodevelopment and unfolded protein response.
HostingRepository	PRIDE
AnnounceDate	2019-12-10
AnnouncementXML	Submission_2020-02-17_02:58:41.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pavel Kielkowski
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-25 00:29:08	ID requested
1	2019-12-10 07:21:06	announced
⏵ 2	2020-02-17 02:58:45	announced	2020-02-17: Updated publication reference for PubMed record(s): 32027064.

Kielkowski P, Buchsbaum IY, Becker T, Bach K, Cappello S, Sieber SA, A Pronucleotide Probe for Live-Cell Imaging of Protein AMPylation. Chembiochem, 21(9):1285-1287(2020) [pubmed]

curator keyword: Technical, Biological

submitter keyword: AMPylation, chemical proteomics

Stepahn A. Sieber
contact affiliation	Technical University of Munich Chair of Organic Chemistry II Lichtenbergstraße 4 D-85748 Garching German
contact email	stephan.sieber@tum.de
lab head
Pavel Kielkowski
contact affiliation	Technical University of Munich, Chair of Organic Chemistry II, Lichtenberstrasse 4, 85764, Garching
contact email	pavel.kielkowski@tum.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/12/PXD016011

PRIDE project URI

• PRIDE
  1. PXD016011
     1. Label: PRIDE project
     2. Name: Pronucleotide probe for in vivo imaging of protein AMPylation.