PXD016010

PXD016010 is an original dataset announced via ProteomeXchange.

Title	Top Down Proteomic analysis of Gingival Crevicular Fluid in deciduous, exfoliating and permanent teeth in children
Description	Gingival Crevicular Fluid, a plasma-derived exudate present in the gingival crevice was collected from deciduous, exfoliating and permanent teeth from 20 children (60 samples) with the aim to characterize and quantify by a mass spectrometry based top-down proteomic approach, the peptide/proteins in the fluid and verify possible variations occurring during the exfoliating process. The results obtained confirmed the presence in Gingival Crevicular Fluid of α-Defensins 1-4, Thymosin β4 and Thymosin β10, as described in previous works and revealed the presence of other interesting peptides never described before in Gingival Crevicular Fluid, such as specific fragments of α-1-antitrypsin, α-1-antichymotrypsin, Thymosin β4 and Thymosin β10 fragments, Fibrinopeptide A, Fibrinopeptide B, S100A, LVV Hemorphin-7 (hemoglobin chain β fragment), as well as some other peptides deriving from α and β subunits of hemoglobin. Statistical analysis evidenced different levels in 5 proteins/peptides in the three groups in particular with higher level in exfoliating teeth. Our study demonstrate that an in-depth analysis of a biological fluid like Gingival Crevicular Fluid, present in small amount, can provide useful information for the understanding of different biological processes like teeth eruption.
HostingRepository	PRIDE
AnnounceDate	2020-07-09
AnnouncementXML	Submission_2020-07-09_02:09:52.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Federica Iavarone
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-24 08:41:31	ID requested
⏵ 1	2020-07-09 02:09:54	announced

Iavarone F, Olianas A, Patini R, Gallenzi P, Di Tonno L, Desiderio C, Cabras T, Manconi B, Vincenzoni F, Cordaro M, Messana I, Urbani A, Castagnola M, Top down proteomic analysis of gingival crevicular fluid in deciduous, exfoliating and permanent teeth in children. J Proteomics, 226():103890(2020) [pubmed]

submitter keyword: proteomics, top Down

Federica Iavarone
contact affiliation	Istituto di Biochimica e Biochimica Clinica, Università Cattolica del Sacro Cuore, Roma, Italia. Fondazione Policlinico Universitario A. Gemelli IRCCS, Roma, Italia.
contact email	federica.iavarone@unicatt.it
lab head
Federica Iavarone
contact affiliation	Biochemistry and Clinical Biochemistry Institute, Medicine Faculty, Catholic University of Rome Fondazione Policlinico Universitario A. Gemelli IRCCS Rome Italy
contact email	federica.iavarone@unicatt.it
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD016010
     1. Label: PRIDE project
     2. Name: Top Down Proteomic analysis of Gingival Crevicular Fluid in deciduous, exfoliating and permanent teeth in children