PXD016008

PXD016008 is an original dataset announced via ProteomeXchange.

Title	Mass spectrometry proteomics detecting interaction proteins of Kindlin-2
Description	We aim to find out the Kindlin-2 interaction proteins. We transfected plasmids Flag or Flag-Kindlin-2 to HKC cell lines and then used Flag-M2 beads to do the coimmunoprecipitation experiments and followed by NuPAGE Tris-Acetate Mini Gels detection and stained by Coomassie brilliant blue. Compared the two groups,and then we will find the proteins interact with Kindlin-2.
HostingRepository	PRIDE
AnnounceDate	2019-12-16
AnnouncementXML	Submission_2019-12-16_01:01:49.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jiagui Song
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-24 06:34:10	ID requested
⏵ 1	2019-12-16 01:01:51	announced

Song J, Wang T, Chi X, Wei X, Xu S, Yu M, He H, Ma J, Li X, Du J, Sun X, Wang Y, Zhan J, Zhang H, Kindlin-2 Inhibits the Hippo Signaling Pathway by Promoting Degradation of MOB1. Cell Rep, 29(11):3664-3677.e5(2019) [pubmed]

submitter keyword: Human renal tubular epithelial cell(HKC)

Hongquan Zhang
contact affiliation	Department of Human Anatomy, Histology, and Embryology, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education) and State Key Laboratory of Natural and Biomimetic Drugs,Peking University Health Science Center, Beijing 100191, China.
contact email	hongquan.zhang@bjmu.edu.cn
lab head
Jiagui Song
contact affiliation	Peking University Health Science Center
contact email	callmelittlegui@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/12/PXD016008

PRIDE project URI

• PRIDE
  1. PXD016008
     1. Label: PRIDE project
     2. Name: Mass spectrometry proteomics detecting interaction proteins of Kindlin-2