PXD016006

PXD016006 is an original dataset announced via ProteomeXchange.

Title	Proteomic and epi/proteomic response of Mycobacterium tuberculosis to nitric oxide stress
Description	Reactive nitrogen species (RNS) are the major antimicrobial molecules secreted by host cells to tackle Mycobacterium tuberculosis (Mtb) infection. Mtb respond and adapt to adverse environmental stressors by changes in gene expression, ensuring their survival. This adaptation process is mediated by a combination of transcriptional regulatory networks that result in altered gene expression and enzyme (protein) activities. Previous studies emphasized primarily on transcriptomic response of Mtb to different environmental stresses, including RNS. In this study, the proteomic response of Mtb to sublethal doses of RNS stress after 30 minutes, 2 hours, 6 hours and 20 hours was explored. We used liquid chromatography coupled with electrospray ionization mass spectrometry (LC-MS/MS) to elucidate the proteomic response of Mtb to RNS. Quantitative proteomic analysis revealed identification of 2911 proteins and 6460 acylations in Mtb. A total of 581 unique proteins were found to be affected by RNS at different time points. Multiple-sample test revealed 297 differentially regulated proteins across the four time points. Furthermore, multiple-sample test identified 127 differentially acylated sites on 94 proteins. Marked changes in proteomic response was observed at later time points, most of which were compensatory responses involved in SOS response, iron homeostasis, energy metabolism and other stress responses. This study provides an improved understanding of the dynamic proteomic response of Mtb to RNS and identification of key molecular switches, which is the basis of controlling bacterial growth in different environmental setting.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:31:36.655.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alemayehu Godana Birhanu
SpeciesList	scientific name: Mycobacterium tuberculosis H37Rv; NCBI TaxID: 83332;
ModificationList	succinylated residue; formylated residue; acetylated residue; complex glycosylation
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-24 06:32:39	ID requested
1	2022-02-15 09:52:42	announced
⏵ 2	2024-10-22 05:31:37	announced	2024-10-22: Updated project metadata.

10.1021/acsomega.1c05923;

Birhanu AG, G, ó, mez-Mu, ñ, oz M, Kalayou S, Riaz T, Lutter T, Yimer SA, Abebe M, T, ø, njum T, Cells Exposed to Nitrosative Stress. ACS Omega, 7(4):3470-3482(2022) [pubmed]

submitter keyword: PTMs, Nitric oxide, proteomics,M. tuberculosis

Alemayehu Godana Birhanu
contact affiliation	PhD candidate, Faculty of Medicine, University of Oslo, Norway
contact email	a.g.birhanu@studmed.uio.no
lab head
Alemayehu Godana Birhanu
contact affiliation	PhD candidate, Faculty of Medicine, University of Oslo, Norway
contact email	alexbiology97@yahoo.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD016006

PRIDE project URI

• PRIDE
  1. PXD016006
     1. Label: PRIDE project
     2. Name: Proteomic and epi/proteomic response of Mycobacterium tuberculosis to nitric oxide stress