PXD016004

PXD016004 is an original dataset announced via ProteomeXchange.

Title	Neuronal proteome dynamics during homeostatic scaling.
Description	Homeostatic scaling adjusts synaptic strength in response to persistent changes in neuronal network activity. This compensatory mechanism requires proteome remodeling accomplished via regulation of protein synthesis as well as degradation, but the global patterns of proteome remodeling and the underlying dynamics of individual proteins remain elusive. Here we used dynamic SILAC labeling in cultured hippocampal cells to identify proteins involved in homeostatic up- or down-scaling and to quantify their changes in synthesis and degradation as well as resulting changes in protein abundance or turnover. Our data demonstrate that a large fraction of the neuronal proteome is remodeled during homeostatic scaling. Most proteins were down-regulated by decreased synthesis or up-regulated by decreased degradation. Comparably fewer proteins showed increased synthesis or degradation rates. More than half of the quantified synaptic proteins were regulated, including pre- as well as postsynaptic proteins with diverse molecular functions.
HostingRepository	PRIDE
AnnounceDate	2020-03-31
AnnouncementXML	Submission_2020-04-07_22:09:01.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Julian Langer
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-24 04:27:38	ID requested
1	2020-03-31 02:39:44	announced
⏵ 2	2020-04-07 22:09:03	announced	2020-04-08: Updated publication reference for PubMed record(s): 32238265.

D, ö, rrbaum AR, Alvarez-Castelao B, Nassim-Assir B, Langer JD, Schuman EM, Proteome dynamics during homeostatic scaling in cultured neurons. Elife, 9():(2020) [pubmed]

curator keyword: Biological

submitter keyword: proteome remodelling, proteome turnover, dynamic SILAC, homeostatic scaling, synaptic plasticity

Julian Langer
contact affiliation	MPIs for Brain Research and Biophysics
contact email	julian.langer@biophys.mpg.de
lab head
Julian Langer
contact affiliation	MPIs for Biophysics and Brain Research
contact email	julian.langer@biophys.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/03/PXD016004

PRIDE project URI

• PRIDE
  1. PXD016004
     1. Label: PRIDE project
     2. Name: Neuronal proteome dynamics during homeostatic scaling.