PXD015991

PXD015991 is an original dataset announced via ProteomeXchange.

Title	SPA-1 attenuate myocardial hypoxia and reoxygenation injury via regulating RAC1: an iTRAQ-based proteomic study
Description	Previous studies indicated that the ras-related C3 botulinum toxin substrate 1 (RAC1), a member of the Rac family of guanosine triphosphate phosphohydrolases (GTPase), is an important regulator of myocardial ischemia and reperfusion (I/R) injury. Potential interaction between the signal-induced proliferation-associated 1 (SPA-1) and RAC1 has been observed in cancer and neurological diseases, but the role of SPA-1 in myocardial I/R injury has not been fully investigated. Using iTRAQ-based proteomic analysis, we found that SPA-1 protein expression was moderately upregulated and RAC1 was significantly activated in H9C2 cells treated with hypoxia and reoxygenation (H/R). Moreover, others have shown that inhibiting SPA-1 with siRNA and overexpressing SPA-1 with plasmid transfection moderately upregulated SPA-1 during H/R which may be protect against H/R related cardiomyocyte injury and subsequent cytoskeleton malformation, cardiac fibrosis, apoptosis, and cellular reactive oxygen species formation. Finally, in a rat model of myocardial I/R induced by left anterior descending artery ligation, adenovirus-mediated overexpression of SPA-1 significantly alleviated the pathohistological changes in the myocardium and inhibited RAC1 activation. Taken together, these results indicate that under I/R conditions, moderate upregulation of SPA-1 may be a self-regulated cardioprotective response against RAC1-mediated injuries via attenuating multiple pathophysiological processes. Targeted enhancing of SPA-1 protein expression and suppressing RAC1 activation may be an important treatment strategy for preventing myocardial I/R injury.
HostingRepository	iProX
AnnounceDate	2019-10-23
AnnouncementXML	Submission_2023-03-01_19:05:12.093.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yinglu Hao
SpeciesList	scientific name: Rattus norvegicus; NCBI TaxID: 10116;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-23 19:52:41	ID requested
1	2019-10-23 20:18:03	announced
⏵ 2	2023-03-01 19:05:12	announced	2023-03-02: Update publication information.

Li C, Li Y, Li Y, Wang Y, Teng Y, Hao Y, activation of RAC1 protein. Mol Med Rep, 27(2):(2023) [pubmed]

submitter keyword: SPA-1, RAC1, myocardium, ischemia and reperfusion injury

Yinglu Hao
contact affiliation	Department of Cardiology
contact email	yingluhaoy@163.com
lab head
Yinglu Hao
contact affiliation	Department of Cardiology
contact email	yingluhaoy@163.com
dataset submitter

iProX dataset URI