PXD015983

PXD015983 is an original dataset announced via ProteomeXchange.

Title	Effects of chronic social stress on mitochondrial and mitochondrial-associated membrane protein networks in the mouse brain
Description	Stressors challenge metabolic processes to adapt to ever-changing environmental conditions and herein, the brain is exceptionally amenable to change. Simultaneously, the high cerebral energy demands may render the brain particularly vulnerable to the metabolic consequences of psychological stress. Mitochondria are central organelles within energy metabolism, known to dynamically adjust function according to shifts in metabolic demands, and thus pivotal in the stress response. It is largely unclear how mitochondria respond to a psychological stressor and how mitochondria orchestrate their actions with other organelles, such as the endoplasmatic reticulum (ER). To address these questions we subjected male mice to chronic social defeat stress (CSD) and analyzed different subcellular brain fractions using label-free proteomics: mitochondrial associated membranes (MAMs), ER, and crude and pure mitochondrial fractions. Although insufficient to warrant a protein pathway analysis, we identified a few noteworthy differentially regulated proteins. These included decreased levels of SH3GL2 (SH3 Domain Containing GRB2 Like 2 or Endophilin 1) in the crude mitochondrial fraction of stressed mice, which may be related to impaired presynaptic calcium influx. In line, CACNA1A (Calcium Voltage-Gated Channel Subunit Alpha1 A) was absent in the pure mitochondrial fraction from CSD-exposed mice, a finding also suggestive of an impaired mitochondrial calcium flux. Then, in the MAM-fraction we observed an absence of HK3 (hexokinase 3) in CSD-treated mice, which may be linked to the CSD-induced cerebral hyperglycemia reported previously. Although verification is still required, our findings revealed a number of differentially expressed proteins possibly critical in the metabolic response to chronic stress.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:52:30.524.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sabine Arndt
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetic acid derivatized residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-23 04:04:04	ID requested
1	2022-10-13 08:04:37	announced
⏵ 2	2023-11-14 07:52:31	announced	2023-11-14: Updated project metadata.

van der Kooij MA, Rojas-Charry L, Givehchi M, Wolf C, Bueno D, Arndt S, Tenzer S, Mattioni L, Treccani G, Hasch A, Schmeisser MJ, Vianello C, Giacomello M, Methner A, Chronic social stress disrupts the intracellular redistribution of brain hexokinase 3 induced by shifts in peripheral glucose levels. J Mol Med (Berl), 100(10):1441-1453(2022) [pubmed]

curator keyword: Biological

submitter keyword: mitochondrial-associated membrane, brain, mouse, chronic stress

Stefan Tenzer
contact affiliation	Insitute of Immunology Univeristy Medical Centre of the Johannes-Gutenberg University Mainz Mainz / Germany
contact email	Tenzer@uni-mainz.de
lab head
Sabine Arndt
contact affiliation	Institute for Immunology, University Medical Center of the Johannes Gutenberg University Mainz, Mainz, Germany
contact email	arndt@uni-mainz.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD015983

PRIDE project URI

• PRIDE
  1. PXD015983
     1. Label: PRIDE project
     2. Name: Effects of chronic social stress on mitochondrial and mitochondrial-associated membrane protein networks in the mouse brain