PXD015982

PXD015982 is an original dataset announced via ProteomeXchange.

Title	Alterations in Extracellular Matrix Composition during Aging and Photoaging of the Skin
Description	Human skin is composed of the cell-rich epidermis, the extracellular matrix (ECM) rich dermis, and the hypodermis. Within the dermis, a dense network of ECM proteins provides structural support to the skin and regulates a wide variety of signaling pathways which govern cell proliferation and other critical processes. Both intrinsic aging, which occurs steadily over time, and extrinsic aging (photoaging), which occurs as a result of external insults such as UV radiation, cause alterations to the dermal ECM. In this study, we utilized both quantitative and global proteomics, alongside single harmonic generation (SHG) and two-photon autofluorescence (TPAF) imaging, to assess changes in dermal composition during intrinsic and extrinsic aging. We find that both intrinsic and extrinsic aging result in significant decreases in structural ECM integrity, evidenced by decreasing collagen abundance and increasing fibril fragmentation, and ECM-supporting proteoglycans. Intrinsic aging also produces changes distinct from those produced by photoaging, including reductions in elastic fiber and crosslinking enzyme abundance. In contrast, photoaging is primarily defined by increases in elastic fiber-associated protein and pro-inflammatory proteases. Changes induced by photoaging are evident even in comparisons of young underarm and forearm skin, indicating that photoexposure experienced by an individual’s mid-20s is sufficient for large-scale proteomic alterations and that molecular-level changes due to photoaging are evident well before clinical indications are present. GO term enrichment revealed that both intrinsic aging and photoaging share common features of chronic inflammation.
HostingRepository	PRIDE
AnnounceDate	2020-07-03
AnnouncementXML	Submission_2020-07-03_00:52:25.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD015982
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Kirk Hansen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Oxidation; Acetyl; Carbamidomethyl; Gln->pyro-Glu
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-23 03:52:14	ID requested
⏵ 1	2020-07-03 00:52:25	announced

Dataset with its publication pending

submitter keyword: Human Skin Proteomics LC-MSMS Aging ECM

Kirk Hansen
contact affiliation	Hansen Lab, Department of Biochemistry and Molecular Genetics, University of Colorado Anschutz Medical Campus, USA
contact email	kirk.hansen@cuanschutz.edu
lab head
Kirk Hansen
contact affiliation	University of Colorado
contact email	kirk.hansen@ucdenver.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD015982
     1. Label: PRIDE project
     2. Name: Alterations in Extracellular Matrix Composition during Aging and Photoaging of the Skin