PXD015980

PXD015980 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of the human milieu of gamete /embryo maternal interaction
Description	The improvement of the embryo culture media is of high relevance due to its influence on successful implantation rates, pregnancy, neonatal outcomes and potential effects in the adult life. The ideal conditions for embryo development are those naturally occurring in the female reproductive tract, i.e., the oviductal and uterine fluids. In order to shed light on the differences between chemical and natural media, we performed the first comparative study of the low abundance proteins in plasma, uterine and oviductal fluid collected, simultaneously, from healthy and fertile women that underwent a salpingectomy. The rationale for this design derives from the fact that high-abundant proteins in these fluids are usually those coming from blood serum and frequently mask the detection of low abundant proteins with a potential significant role on specific processes related to the embryo-maternal interaction. The proteomic analysis by 1D-nano LC ESI-MSMS detected several proteins in higher amounts in oviductal fluid when compared to uterine and plasma samples (RL3, GSTA1, EZRI, DPYSL3, GARS, HSP90A). Such oviductal fluid proteins could be a target to improve fertilization rates and early embryo development, if used in the culture media. In conclusion, this study presents a high-throughput analysis of female reproductive tract fluids and contributes to the knowledge of oviductal and uterine secretome.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_03:59:14.371.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alberto Paradela
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-23 01:29:21	ID requested
1	2019-10-29 03:29:50	announced
⏵ 2	2024-10-22 03:59:22	announced	2024-10-22: Updated project metadata.

10.3390/ijms20215305;

Canha-Gouveia A, Paradela A, Ramos-Fern, á, ndez A, Prieto-S, á, nchez MT, S, á, nchez-Ferrer ML, Corrales F, Coy P, Which Low-Abundance Proteins are Present in the Human Milieu of Gamete/Embryo Maternal Interaction? Int J Mol Sci, 20(21):(2019) [pubmed]

submitter keyword: low abundance proteins

human reproductive fluids

salpingectomy

Pilar Coy
contact affiliation	Department of Physiology, Faculty of Veterinary, University of Murcia, Campus Mare Nostrum, IMIB-Arrixaca, Murcia, Spain
contact email	pcoy@um.es
lab head
Alberto Paradela
contact affiliation	Proteomics Lab CNB-CSIC
contact email	alberto.paradela@cnb.csic.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/10/PXD015980

PRIDE project URI

• PRIDE
  1. PXD015980
     1. Label: PRIDE project
     2. Name: Proteomic analysis of the human milieu of gamete /embryo maternal interaction