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PXD015980

PXD015980 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomic analysis of the human milieu of gamete /embryo maternal interaction
DescriptionThe improvement of the embryo culture media is of high relevance due to its influence on successful implantation rates, pregnancy, neonatal outcomes and potential effects in the adult life. The ideal conditions for embryo development are those naturally occurring in the female reproductive tract, i.e., the oviductal and uterine fluids. In order to shed light on the differences between chemical and natural media, we performed the first comparative study of the low abundance proteins in plasma, uterine and oviductal fluid collected, simultaneously, from healthy and fertile women that underwent a salpingectomy. The rationale for this design derives from the fact that high-abundant proteins in these fluids are usually those coming from blood serum and frequently mask the detection of low abundant proteins with a potential significant role on specific processes related to the embryo-maternal interaction. The proteomic analysis by 1D-nano LC ESI-MSMS detected several proteins in higher amounts in oviductal fluid when compared to uterine and plasma samples (RL3, GSTA1, EZRI, DPYSL3, GARS, HSP90A). Such oviductal fluid proteins could be a target to improve fertilization rates and early embryo development, if used in the culture media. In conclusion, this study presents a high-throughput analysis of female reproductive tract fluids and contributes to the knowledge of oviductal and uterine secretome.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_03:59:14.371.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAlberto Paradela
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentTripleTOF 5600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-10-23 01:29:21ID requested
12019-10-29 03:29:50announced
22024-10-22 03:59:22announced2024-10-22: Updated project metadata.
Publication List
10.3390/ijms20215305;
Canha-Gouveia A, Paradela A, Ramos-Fern, á, ndez A, Prieto-S, á, nchez MT, S, á, nchez-Ferrer ML, Corrales F, Coy P, Which Low-Abundance Proteins are Present in the Human Milieu of Gamete/Embryo Maternal Interaction? Int J Mol Sci, 20(21):(2019) [pubmed]
Keyword List
submitter keyword: low abundance proteins
human reproductive fluids
salpingectomy
Contact List
Pilar Coy
contact affiliationDepartment of Physiology, Faculty of Veterinary, University of Murcia, Campus Mare Nostrum, IMIB-Arrixaca, Murcia, Spain
contact emailpcoy@um.es
lab head
Alberto Paradela
contact affiliationProteomics Lab CNB-CSIC
contact emailalberto.paradela@cnb.csic.es
dataset submitter
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