PXD015980 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic analysis of the human milieu of gamete /embryo maternal interaction |
Description | The improvement of the embryo culture media is of high relevance due to its influence on successful implantation rates, pregnancy, neonatal outcomes and potential effects in the adult life. The ideal conditions for embryo development are those naturally occurring in the female reproductive tract, i.e., the oviductal and uterine fluids. In order to shed light on the differences between chemical and natural media, we performed the first comparative study of the low abundance proteins in plasma, uterine and oviductal fluid collected, simultaneously, from healthy and fertile women that underwent a salpingectomy. The rationale for this design derives from the fact that high-abundant proteins in these fluids are usually those coming from blood serum and frequently mask the detection of low abundant proteins with a potential significant role on specific processes related to the embryo-maternal interaction. The proteomic analysis by 1D-nano LC ESI-MSMS detected several proteins in higher amounts in oviductal fluid when compared to uterine and plasma samples (RL3, GSTA1, EZRI, DPYSL3, GARS, HSP90A). Such oviductal fluid proteins could be a target to improve fertilization rates and early embryo development, if used in the culture media. In conclusion, this study presents a high-throughput analysis of female reproductive tract fluids and contributes to the knowledge of oviductal and uterine secretome. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_03:59:14.371.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Alberto Paradela |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue |
Instrument | TripleTOF 5600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-10-23 01:29:21 | ID requested | |
1 | 2019-10-29 03:29:50 | announced | |
⏵ 2 | 2024-10-22 03:59:22 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.3390/ijms20215305; |
Canha-Gouveia A, Paradela A, Ramos-Fern, á, ndez A, Prieto-S, á, nchez MT, S, á, nchez-Ferrer ML, Corrales F, Coy P, Which Low-Abundance Proteins are Present in the Human Milieu of Gamete/Embryo Maternal Interaction? Int J Mol Sci, 20(21):(2019) [pubmed] |
Keyword List
submitter keyword: low abundance proteins |
human reproductive fluids |
salpingectomy |
Contact List
Pilar Coy |
contact affiliation | Department of Physiology, Faculty of Veterinary, University of Murcia, Campus Mare Nostrum, IMIB-Arrixaca, Murcia, Spain |
contact email | pcoy@um.es |
lab head | |
Alberto Paradela |
contact affiliation | Proteomics Lab CNB-CSIC |
contact email | alberto.paradela@cnb.csic.es |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD015980
- Label: PRIDE project
- Name: Proteomic analysis of the human milieu of gamete /embryo maternal interaction