PXD015977

PXD015977 is an original dataset announced via ProteomeXchange.

Title	An ‘omics approach to unraveling the paradoxical effect of diet on perfluorooctanesulfonic acid (PFOS) and perfluorononanoic acid (PFNA) induced non-alcoholic fatty liver disease (NAFLD)
Description	Perfluoroalkyl substances (PFASs) are a family of toxicants universally detected in human serum and known to cause dyslipidemia in animals and humans. Non-alcoholic fatty liver disease (NAFLD) is most prevalent form of liver disease in the United States and has been rising in global prevalence over recent years. This study explored diet-PFAS interactions and their potential role in the increasing global incidence of NAFLD. Male C57BL/6 mice were fed with either a low-fat diet (10% kcal from fat) or a moderately high fat diet (45% kcal from fat) with or without perfluorooctanesulfonic acid (PFOS) or perfluorononanoic acid (PFNA) at a low dose of 0.0003% w/w in feed for 12 weeks. Livers were excised for histology and quantification of PFASs and lipids. Proteomics and transcriptomics were utilized to conduct mechanistic pathway exploration. In a high fat diet, PFOS and PFNA protected against the onset of hepatic lipid accumulation and inflammatory progression. Genes and proteins related to lipid metabolism, synthesis, transport, and storage were modulated by PFAS exposure and further impacted by the presence of dietary fat. When combined with a high fat diet, low dose PFOS and PFNA are protective against the onset of NAFLD suggesting that dietary fat impacts the behavior of PFASs in vivo. Furthermore, both dietary fat content and the chemical functional head group exerted significant influence on tissue partitioning and the resulting hepatic biochemical signature.
HostingRepository	PRIDE
AnnounceDate	2021-02-18
AnnouncementXML	Submission_2021-02-18_15:47:26.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marisa Pfohl
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-23 01:29:03	ID requested
⏵ 1	2021-02-18 15:47:26	announced

Dataset with its publication pending

submitter keyword: Fatty Liver Disease, PFNA, PFOS, NAFLD, High Fat Diet, Hepatotoxicity

Dr. Angela Slitt
contact affiliation	Department of Biomedical and Pharmaceutical Sciences, University of Rhode Island, Kingston, RI USA
contact email	aslitt@uri.edu
lab head
Marisa Pfohl
contact affiliation	University of Rhode Island
contact email	marisa_pfohl@uri.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD015977
     1. Label: PRIDE project
     2. Name: An ‘omics approach to unraveling the paradoxical effect of diet on perfluorooctanesulfonic acid (PFOS) and perfluorononanoic acid (PFNA) induced non-alcoholic fatty liver disease (NAFLD)