PXD015975

PXD015975 is an original dataset announced via ProteomeXchange.

Title	Host-defence peptides caerin 1.1 and 1.9 stimulate TNF-alpha-dependent apoptosis in human cervical cancer HeLa cells
Description	TNFα pathway has been recognised as one of the most important signalling pathways characterising molecules with anti-cancer activity, due to it closely relates to cell survival as well as apoptosis. Isolated from the glandular secretion of Australian tree frogs, the genus Litoria, host-defence caerin peptides have been previously shown to have multiple biological activities, including anti-proliferation of several cancer cell lines, though the mechanism remains unclear. In this study, we used multiple immunoassays, confocal microscopy in conjunction with TMT-labelling quantitative proteomics to investigate the anti-proliferative activities of caerin 1.1 and 1.9 against cervical cancer HeLa cells. The proteins of cells and cell growth environment post the treatments were comparatively analysed, respectively. We found that both caerin 1.1 and 1.9 highly inhibited HeLa cell proliferation with a significant additive effect. The PPI analysis confirmed that the interactions among the significantly regulated proteins were highly intensive. Besides translation, folding and localisation of proteins and RNA processing, apoptosis process was significantly enriched post the treatments. The apoptosis process was resulted by TNFα signalling, confirmed by the elevated levels of CASP3 and CASP9. Moreover, EGFR1 and androgen receptor pathways appeared inhibited. The activation of TCR pathway derived from the quantitation results further implies the likelihood of recruiting more T cells to the cell growth environment post the treatment and more sensitive to T cell mediated killing of Hela cells.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:11:26.065.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tianfang Wang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	X500R QTOF

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-23 01:28:55	ID requested
1	2020-08-26 23:11:32	announced
2	2020-09-01 00:19:32	announced	2020-09-01: Updated publication reference for PubMed record(s): 32850805.
⏵ 3	2024-10-22 05:11:29	announced	2024-10-22: Updated project metadata.

Ni G, Chen S, Chen M, Wu J, Yang B, Yuan J, Walton SF, Li H, Wei MQ, Wang Y, Chen G, Liu X, Wang T, Host-Defense Peptides Caerin 1.1 and 1.9 Stimulate TNF-Alpha-Dependent Apoptotic Signals in Human Cervical Cancer HeLa Cells. Front Cell Dev Biol, 8():676(2020) [pubmed]

10.3389/fcell.2020.00676;

submitter keyword: proteomics, HeLa cell, TMT labelling, TNFα signalling pathway,caerin peptide, apoptosis, confocal microscopy, western blot

Tianfang Wang
contact affiliation	Genecology Research Centre, University of the Sunshine Coast, Maroochydore DC, QLD 4558, Australia
contact email	twang@usc.edu.au
lab head
Tianfang Wang
contact affiliation	University of the Sunshine Coast
contact email	twang@usc.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD015975
     1. Label: PRIDE project
     2. Name: Host-defence peptides caerin 1.1 and 1.9 stimulate TNF-alpha-dependent apoptosis in human cervical cancer HeLa cells