PXD015960

PXD015960 is an original dataset announced via ProteomeXchange.

Title	Proteome profile of peripheral myelin in healthy mice and in a neuropathy model
Description	Using the sciatic nerve as a model, we biochemically purified peripheral nervous system (PNS) myelin and systematically assessed its proteome by dedicated, partly ion mobility-enhanced data-independent acquisition (DIA) workflows with alternating low and elevated energy (MSE) modes. Specifically, we used MSE for correct quantification of highest-abundance proteins, UDMSE for deep quantitative proteome coverage, and dynamic range enhancement (DRE)-UDMSE for comparative analysis, the latter mode providing a good compromise between dynamic range, identification rate and instrument run time for routine differential myelin proteome profiling. We found that integration of the proteome data with the total nerve transcriptome is suited to determine comprehensive molecular profiles in healthy nerves and in myelin-related disorders. This work provides the most comprehensive proteome resource thus far to approach development, function and pathology of peripheral myelin, and a straightforward, accurate and sensitive workflow to address myelin diversity in health and disease.
HostingRepository	PRIDE
AnnounceDate	2020-02-20
AnnouncementXML	Submission_2020-05-18_06:45:05.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Olaf Jahn
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-22 05:27:30	ID requested
1	2020-02-20 13:14:23	announced
⏵ 2	2020-05-18 06:45:06	announced	2020-05-18: Updated publication reference for PubMed record(s): 32130108.

Siems SB, Jahn O, Eichel MA, Kannaiyan N, Wu LMN, Sherman DL, Kusch K, Hesse D, Jung RB, Fledrich R, Sereda MW, Rossner MJ, Brophy PJ, Werner HB, Proteome profile of peripheral myelin in healthy mice and in a neuropathy model. Elife, 9():(2020) [pubmed]

curator keyword: Biomedical

submitter keyword: Mouse, peripheral nervous system (PNS), myelin proteome, label-free quantification, dynamic range enhancement, transcriptome, neuropathy, Charcot-Marie-Tooth disease, demyelination, axon degeneration

Olaf Jahn
contact affiliation	PD Dr. Olaf Jahn Proteomics Group Max-Planck-Institute of Experimental Medicine Hermann-Rein-Str. 3 37075 Goettingen, Germany Tel: +49-551-3899313 Fax: +49-551-3899323 E-mail: jahn@em.mpg.de URL: www.em.mpg.de/proteomics
contact email	jahn@em.mpg.de
lab head
Olaf Jahn
contact affiliation	Proteomics Group, Max-Planck-Institute of Experimental Medicine, Göttingen
contact email	jahn@em.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/02/PXD015960

PRIDE project URI

• PRIDE
  1. PXD015960
     1. Label: PRIDE project
     2. Name: Proteome profile of peripheral myelin in healthy mice and in a neuropathy model