PXD015958

PXD015958 is an original dataset announced via ProteomeXchange.

Title	Plasma proteomic profiling of normal and over-conditioned dairy cows during the transition from late pregnancy to early lactation by LC-MS/MS
Description	The objective of this study was to apply a quantitative proteomic approach to investigate changes in the plasma proteome of normal and over-conditioned dairy cows during the transition period. We used an animal model with a targeted difference in body condition score (BCS) at calving. For this, multiparous Holstein cows were allocated to two groups 15 weeks before anticipated calving and were fed differently to reach either high (HBCS: 7.2 NEL MJ/kg dry matter (DM) or normal BCS (NBCS: 6.8 NEL MJ/kg DM) until dry-off. Thereafter, both groups were fed identical diets. The targeted BCS difference was sustained during the dry period (7 weeks before calving) and the subsequent 12 weeks of lactation. Plasma samples (n = 5) from d -49, +7, and +21 relative to parturition were used from both groups (each n = 5) for proteome profiling in aby applying quantitative Tandem Mass Tags (TMT) approach.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:02:06.997.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Anita Horvatic
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-22 03:07:57	ID requested
1	2020-04-15 22:41:47	announced
⏵ 2	2024-10-22 05:02:07	announced	2024-10-22: Updated project metadata.

Ghaffari MH, Schuh K, Kule, š J, Guillemin N, Horvati, ć A, Mrljak V, Eckersall PD, Dusel G, Koch C, Sadri H, Sauerwein H, Plasma proteomic profiling and pathway analysis of normal and overconditioned dairy cows during the transition from late pregnancy to early lactation. J Dairy Sci, 103(5):4806-4821(2020) [pubmed]

10.3168/jds.2019-17897;

submitter keyword: TMT proteomics,Body condition score, transition cows

Peter David Eckersall
contact affiliation	VetMedZg Laboratory,Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Institute of Biodiversity, Animal Health & Comparative Medicine, College of Medicine, Veterinary Medicine and Life Sciences, University of Glasgow, Glasgow, UK
contact email	David.Eckersall@glasgow.ac.uk
lab head
Anita Horvatic
contact affiliation	University of Zagreb, Faculty of Veterinary Medicine
contact email	horvatic.ani@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/04/PXD015958

PRIDE project URI

• PRIDE
  1. PXD015958
     1. Label: PRIDE project
     2. Name: Plasma proteomic profiling of normal and over-conditioned dairy cows during the transition from late pregnancy to early lactation by LC-MS/MS