PXD015951

PXD015951 is an original dataset announced via ProteomeXchange.

Title	Canine pyometra - plasma proteomics
Description	Canine pyometra is a common inflammatory disease of the uterus in sexually mature bitches, caused by a secondary bacterial infection which leads to change in the plasma protein associated with the innate immune system. Proteomic offers a means to determine the profile of plasma in dogs with pyometra to provide important findings into general mechanisms operating during diverse inflammatory reactions. The plasma protein profile of healthy and pyometra affected bitches was determined by means of an isobaric tandem mass tag (TMT) label-based high-resolution quantitative proteomic approach. Six clinically healthy dogs (used as control group) and 6 dogs with pyometra were enrolled in the study. All dogs were admitted to the Small Animal Clinic, University of Veterinary Medicine Hannover and treated in accordance with the German Animal Welfare Law. The experimental design was approved by the Animal Welfare officer of the University of Veterinary Medicine and by the Ethic Committee of the responsible authority (Lower Saxony State Office for Consumer Protection and Food Safety, reference number 17A 101). Healthy dogs for blood collection were recruited by launching a call in the University of Veterinary Medicine Hannover network for students and staff members for haemostasis study, providing a free clinical and laboratory health check of their animal in combination with the blood collection. Residual sample material was used in the present study.
HostingRepository	PRIDE
AnnounceDate	2020-05-18
AnnouncementXML	Submission_2020-05-18_00:54:40.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Anita Horvatic
SpeciesList	scientific name: Canis lupus familiaris; NCBI TaxID: 9615;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-22 01:25:37	ID requested
⏵ 1	2020-05-18 00:54:41	announced

Kule, š J, Horvati, ć A, Guillemin N, Ferreira RF, Mischke R, Mrljak V, Chadwick CC, Eckersall PD, The plasma proteome and the acute phase protein response in canine pyometra. J Proteomics, 223():103817(2020) [pubmed]

submitter keyword: Dog, pyometra, proteomics, serum, TMT

Peter David Eckersall
contact affiliation	VetMedZg Laboratory,Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia Institute of Biodiversity, Animal Health & Comparative Medicine, College of Medicine, Veterinary Medicine and Life Sciences, University of Glasgow, Glasgow, UK
contact email	David.Eckersall@glasgow.ac.uk
lab head
Anita Horvatic
contact affiliation	University of Zagreb, Faculty of Veterinary Medicine
contact email	horvatic.ani@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD015951

PRIDE project URI

• PRIDE
  1. PXD015951
     1. Label: PRIDE project
     2. Name: Canine pyometra - plasma proteomics