PXD015940

PXD015940 is an original dataset announced via ProteomeXchange.

Title	Evaluation of NHS-Acetate and DEPC labelling for determination of solvent accessible amino acid residues in protein complexes
Description	The structural analysis of proteins and protein complexes is key for understanding their function and regulation. In order to gain structural information, we examined protein surface labelling using NHS-Acetate and DEPC following an MS-based workflow. Using two model proteins we characterise the obtained mass spectra and explore the applicability of a quantitative approach for determining of solvent accessible amino acid residues on the surface of proteins and protein complexes. The modified peptides were analysed by LC-MS/MS and processed using MaxQuant for identification and intensity determination of labelled and unlabelled peptides.
HostingRepository	PRIDE
AnnounceDate	2020-05-05
AnnouncementXML	Submission_2020-05-06_00:51:33.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marie Barth
SpeciesList	scientific name: Oryctolagus cuniculus cuniculus; NCBI TaxID: 568996; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-21 05:35:04	ID requested
1	2020-05-05 01:16:01	announced
⏵ 2	2020-05-06 00:51:34	announced	2020-05-06: Updated publication reference for PubMed record(s): 32348883.

Barth M, Bender J, Kundlacz T, Schmidt C, Evaluation of NHS-Acetate and DEPC labelling for determination of solvent accessible amino acid residues in protein complexes. J Proteomics, 222():103793(2020) [pubmed]

submitter keyword: Chemical labelling, mass spectrometry, N-hydroxysuccinimidyl acetate, diethylpyrocarbonate, neutral loss, labelling percentage, solvent accessible surface area

Carla Schmidt
contact affiliation	Interdisciplinary research centre HALOmem, Charles Tanford Protein Center, Institute for Biochemistry and Biotechnology, Martin Luther University Halle-Wittenberg, Halle, Germany
contact email	carla.schmidt@biochemtech.uni-halle.de
lab head
Marie Barth
contact affiliation	HALOmem, Martin Luther University Halle-Wittenberg
contact email	marie.barth@bct.uni-halle.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD015940
     1. Label: PRIDE project
     2. Name: Evaluation of NHS-Acetate and DEPC labelling for determination of solvent accessible amino acid residues in protein complexes