PXD015940

PXD015940 is an original dataset announced via ProteomeXchange.

Title	Evaluation of NHS-Acetate and DEPC labelling for determination of solvent accessible amino acid residues in protein complexes
Description	The structural analysis of proteins and protein complexes is key for understanding their function and regulation. In order to gain structural information, we examined protein surface labelling using NHS-Acetate and DEPC following an MS-based workflow. Using two model proteins we characterise the obtained mass spectra and explore the applicability of a quantitative approach for determining of solvent accessible amino acid residues on the surface of proteins and protein complexes. The modified peptides were analysed by LC-MS/MS and processed using MaxQuant for identification and intensity determination of labelled and unlabelled peptides.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:03:11.843.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marie Alfes
SpeciesList	scientific name: Oryctolagus cuniculus cuniculus; NCBI TaxID: 568996; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-21 05:35:04	ID requested
1	2020-05-05 01:16:01	announced
2	2020-05-06 00:51:34	announced	2020-05-06: Updated publication reference for PubMed record(s): 32348883.
⏵ 3	2024-10-22 05:03:12	announced	2024-10-22: Updated project metadata.

10.1016/j.jprot.2020.103793;

Barth M, Bender J, Kundlacz T, Schmidt C, Evaluation of NHS-Acetate and DEPC labelling for determination of solvent accessible amino acid residues in protein complexes. J Proteomics, 222():103793(2020) [pubmed]

submitter keyword: diethylpyrocarbonate, N-hydroxysuccinimidyl acetate, mass spectrometry, neutral loss,Chemical labelling, solvent accessible surface area, labelling percentage

Carla Schmidt
contact affiliation	Interdisciplinary research centre HALOmem, Charles Tanford Protein Center, Institute for Biochemistry and Biotechnology, Martin Luther University Halle-Wittenberg, Halle, Germany
contact email	carla.schmidt@biochemtech.uni-halle.de
lab head
Marie Alfes
contact affiliation	HALOmem, Martin Luther University Halle-Wittenberg
contact email	marie.barth@bct.uni-halle.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD015940

PRIDE project URI

• PRIDE
  1. PXD015940
     1. Label: PRIDE project
     2. Name: Evaluation of NHS-Acetate and DEPC labelling for determination of solvent accessible amino acid residues in protein complexes