PXD015937

PXD015937 is an original dataset announced via ProteomeXchange.

Title	The small ORF trpM stimulates growth and actinorhodin production in Streptomyces coelicolor
Description	In actinomycetes, antibiotic production is often associated with a morpho-physiological differentiation program that is regulated by complex molecular and metabolic networks. Many aspects of these regulatory circuits have been already elucidated and many others still deserve further investigations. In this regard, the possible role of many small open-reading frames(smORFs) in actinomycete morpho-physiological differentiation is still elusive. In Streptomyces coelicolor, inactivation of the smORF trpM (SCO2038) - whose product modulates L-tryptophan metabolism - impairs production of antibiotics and morphological differentiation. Indeed, it was demonstrated that TrpM is able to interact with PepA (SCO2179), a putative cytosol aminopeptidase playing a key role in antibiotic production and sporulation. In this work, a S. coelicolor trpM knock-in (Sco-trpMKI) mutant strain was generated by cloning trpM into overexpressing vector to further investigate the role of trpM in actinomycete growth and morpho-physiological differentiation. Results highlighted that trpM: i) stimulates growth and actinorhodin production; ii) decreases calcium-dependent antibiotic production; iii) has no effect on undecylprodigiosin production. Metabolic pathways influenced by trpM knock-in were investigated by combining two-difference in gel electrophoresis/nanoliquid chromatography coupled to electrospray linear ion trap tandem mass spectrometry (2D-DIGE/nanoLC-ESI-LIT- MS/MS) and by LC-ESI-MS/MS procedures, respectively. These analyses demonstrated that over-expression of trpM causes an over-representation of factors involved in protein synthesis and nucleotide metabolism as well as a down-representation of proteins involved in central carbon and amino acid metabolism. At the metabolic level, this corresponded to a differential accumulation pattern of different amino acids - including aromatic ones but tryptophan – and central carbon intermediates. PepA was also down-represented in Sco-trpMKI. The latter was produced as recombinant His- tagged protein and was originally proven having the predicted aminopeptidase activity. Altogether, these results highlight the stimulatory effect of trpM in S. coelicolor growth and actinorhodin biosynthesis, which are elicited through the modulation of various metabolic pathways and PepA representation, further confirming the complexity of regulatory networks that control antibiotic production in actinomycetes.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:05:03.931.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Giovanni Renzone
SpeciesList	scientific name: Streptomyces coelicolor; NCBI TaxID: 1902;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; Pyro-carbamidomethyl
Instrument	LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-21 04:08:22	ID requested
1	2020-03-27 04:29:21	announced
⏵ 2	2024-10-22 04:05:14	announced	2024-10-22: Updated project metadata.

10.3389/fmicb.2020.00224;

Vassallo A, Palazzotto E, Renzone G, Botta L, Faddetta T, Scaloni A, Puglia AM, Gallo G, Stimulates Growth and Morphological Development and Exerts Opposite Effects on Actinorhodin and Calcium-Dependent Antibiotic Production. Front Microbiol, 11():224(2020) [pubmed]

10.3390/proteomes8010003;

Ye Z, Sangireddy SR, Yu CL, Hui D, Howe K, Fish T, Thannhauser TW, Zhou S, Comparative Proteomics of Root Apex and Root Elongation Zones Provides Insights into Molecular Mechanisms for Drought Stress and Recovery Adjustment in Switchgrass. Proteomes, 8(1):(2020) [pubmed]

submitter keyword: Streptomyces coelicolor, primary and secondary metabolism, calcium-dependent antibiotic (CDA), Actinorhodin production, cytosol aminopeptidase,small ORF

Anna Maria Puglia
contact affiliation	Laboratory of Molecular Microbiology and Biotechnology, STEBICEF Department, University of 8 Palermo, 90128 Palermo, Italy.
contact email	a.maria.puglia@unipa.it
lab head
Giovanni Renzone
contact affiliation	ISPAAM-CNR
contact email	giovanni.renzone@cnr.it
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD015937
     1. Label: PRIDE project
     2. Name: The small ORF trpM stimulates growth and actinorhodin production in Streptomyces coelicolor