PXD015921

PXD015921 is an original dataset announced via ProteomeXchange.

Title	Circulating Exosomes proteome of Colorectal Cancer Based on Rapid DIA-MS
Description	Early screening is essential for the treatment of colorectal cancer (CRC). Concerns are rising regarding liquid biopsy due to its low invasiveness and reproducibility, especially when tissue samples are not available. Recent reports suggest that tumor-specific proteins may be stably expressed in exosomes. Here we isolated exosomes from the plasma of CRC patients from different stages by prolonged ultracentrifugation combined with sucrose density gradient centrifugation. We performed proteomics and phosphoproteomics analysis of these exosomes using TMT-labeled LC-MS/MS, and found that fibronectin 1(FN1), haptoglobin (HP), S100A9, fibrinogen α chain (FGA) and their phosphorylation levels associated significantly with tumor progression. After verified this result by western blot (WB) in both another cohort and in mouse subcutaneous tumor model, we confirmed the difference among healthy individuals and patients diagnosed with colon adenoma or CRC by optimized Data Independent Acquisition (DIA) mass spectrometry. Our data suggest that rapid DIA mass spectrometry-based liquid biopsy of FGA+ circulating exosomes (crExos) could serve as a potential non-invasive diagnostic and screening tool to identify early stages of CRC.
HostingRepository	iProX
AnnounceDate	2019-10-20
AnnouncementXML	Submission_2021-05-06_01:01:35.328.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xi Zheng
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-20 19:20:36	ID requested
⏵ 1	2021-05-06 01:01:46	announced

Zheng X, Xu K, Zhou B, Chen T, Huang Y, Li Q, Wen F, Ge W, Wang J, Yu S, Sun L, Zhu L, Liu W, Gao H, Yue L, Cai X, Zhang Q, Ruan G, Zhu T, Wu Z, Zhu Y, Shao Y, Guo T, Zheng S, A circulating extracellular vesicles-based novel screening tool for colorectal cancer revealed by shotgun and data-independent acquisition mass spectrometry. J Extracell Vesicles, 9(1):1750202(2020) [pubmed]

submitter keyword: Colorectal Cancer, Exosome, Proteome, Phosphoproteome, Biomarker

Shu Zheng
contact affiliation	Cancer Institute (Key Laboratory of Cancer Prevention and Intervention, China National Ministry of Education), The Second Affiliated Hospital, Zhejiang University School of Medicine
contact email	zhengshu@zju.edu.cn
lab head
Xi Zheng
contact affiliation	Cancer Institute (Key Laboratory of Cancer Prevention and Intervention, China National Ministry of Education), The Second Affiliated Hospital, Zhejiang University School of Medicine
contact email	zhengxisue@zju.edu.cn
dataset submitter

iProX dataset URI