PXD015921
PXD015921 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Circulating Exosomes proteome of Colorectal Cancer Based on Rapid DIA-MS |
Description | Early screening is essential for the treatment of colorectal cancer (CRC). Concerns are rising regarding liquid biopsy due to its low invasiveness and reproducibility, especially when tissue samples are not available. Recent reports suggest that tumor-specific proteins may be stably expressed in exosomes. Here we isolated exosomes from the plasma of CRC patients from different stages by prolonged ultracentrifugation combined with sucrose density gradient centrifugation. We performed proteomics and phosphoproteomics analysis of these exosomes using TMT-labeled LC-MS/MS, and found that fibronectin 1(FN1), haptoglobin (HP), S100A9, fibrinogen α chain (FGA) and their phosphorylation levels associated significantly with tumor progression. After verified this result by western blot (WB) in both another cohort and in mouse subcutaneous tumor model, we confirmed the difference among healthy individuals and patients diagnosed with colon adenoma or CRC by optimized Data Independent Acquisition (DIA) mass spectrometry. Our data suggest that rapid DIA mass spectrometry-based liquid biopsy of FGA+ circulating exosomes (crExos) could serve as a potential non-invasive diagnostic and screening tool to identify early stages of CRC. |
HostingRepository | iProX |
AnnounceDate | 2019-10-20 |
AnnouncementXML | Submission_2021-05-06_01:01:35.328.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Xi Zheng |
SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2019-10-20 19:20:36 | ID requested | |
⏵ 1 | 2021-05-06 01:01:46 | announced |
Publication List
Zheng X, Xu K, Zhou B, Chen T, Huang Y, Li Q, Wen F, Ge W, Wang J, Yu S, Sun L, Zhu L, Liu W, Gao H, Yue L, Cai X, Zhang Q, Ruan G, Zhu T, Wu Z, Zhu Y, Shao Y, Guo T, Zheng S, A circulating extracellular vesicles-based novel screening tool for colorectal cancer revealed by shotgun and data-independent acquisition mass spectrometry. J Extracell Vesicles, 9(1):1750202(2020) [pubmed] |
Keyword List
submitter keyword: Colorectal Cancer, Exosome, Proteome, Phosphoproteome, Biomarker |
Contact List
Shu Zheng | |
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contact affiliation | Cancer Institute (Key Laboratory of Cancer Prevention and Intervention, China National Ministry of Education), The Second Affiliated Hospital, Zhejiang University School of Medicine |
contact email | zhengshu@zju.edu.cn |
lab head | |
Xi Zheng | |
contact affiliation | Cancer Institute (Key Laboratory of Cancer Prevention and Intervention, China National Ministry of Education), The Second Affiliated Hospital, Zhejiang University School of Medicine |
contact email | zhengxisue@zju.edu.cn |
dataset submitter |
Full Dataset Link List
iProX dataset URI |