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PXD015921

PXD015921 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCirculating Exosomes proteome of Colorectal Cancer Based on Rapid DIA-MS
DescriptionEarly screening is essential for the treatment of colorectal cancer (CRC). Concerns are rising regarding liquid biopsy due to its low invasiveness and reproducibility, especially when tissue samples are not available. Recent reports suggest that tumor-specific proteins may be stably expressed in exosomes. Here we isolated exosomes from the plasma of CRC patients from different stages by prolonged ultracentrifugation combined with sucrose density gradient centrifugation. We performed proteomics and phosphoproteomics analysis of these exosomes using TMT-labeled LC-MS/MS, and found that fibronectin 1(FN1), haptoglobin (HP), S100A9, fibrinogen α chain (FGA) and their phosphorylation levels associated significantly with tumor progression. After verified this result by western blot (WB) in both another cohort and in mouse subcutaneous tumor model, we confirmed the difference among healthy individuals and patients diagnosed with colon adenoma or CRC by optimized Data Independent Acquisition (DIA) mass spectrometry. Our data suggest that rapid DIA mass spectrometry-based liquid biopsy of FGA+ circulating exosomes (crExos) could serve as a potential non-invasive diagnostic and screening tool to identify early stages of CRC.
HostingRepositoryiProX
AnnounceDate2019-10-20
AnnouncementXMLSubmission_2021-05-06_01:01:35.328.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterXi Zheng
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-10-20 19:20:36ID requested
12021-05-06 01:01:46announced
Publication List
Zheng X, Xu K, Zhou B, Chen T, Huang Y, Li Q, Wen F, Ge W, Wang J, Yu S, Sun L, Zhu L, Liu W, Gao H, Yue L, Cai X, Zhang Q, Ruan G, Zhu T, Wu Z, Zhu Y, Shao Y, Guo T, Zheng S, A circulating extracellular vesicles-based novel screening tool for colorectal cancer revealed by shotgun and data-independent acquisition mass spectrometry. J Extracell Vesicles, 9(1):1750202(2020) [pubmed]
Keyword List
submitter keyword: Colorectal Cancer, Exosome, Proteome, Phosphoproteome, Biomarker
Contact List
Shu Zheng
contact affiliationCancer Institute (Key Laboratory of Cancer Prevention and Intervention, China National Ministry of Education), The Second Affiliated Hospital, Zhejiang University School of Medicine
contact emailzhengshu@zju.edu.cn
lab head
Xi Zheng
contact affiliationCancer Institute (Key Laboratory of Cancer Prevention and Intervention, China National Ministry of Education), The Second Affiliated Hospital, Zhejiang University School of Medicine
contact emailzhengxisue@zju.edu.cn
dataset submitter
Full Dataset Link List
iProX dataset URI