PXD015920

PXD015920 is an original dataset announced via ProteomeXchange.

Title	The inner nuclear membrane-associated degradation system in plants
Description	The homeostasis of the inner nuclear membrane (INM) proteins plays a critical role in regulating the nuclear structure, genome integrity, and signal transduction. However, little is known about the turnover of INM proteins in higher eukaryotes. Here we demonstrated that the conserved INM protein SUN1 undergoes proteasome-dependent degradation in Arabidopsis. Using in vivo proximity labeling-coupled mass spectrometry, we showed that SUN1 associates with the CDC48 complex, which mediates membrane protein extraction and proteolysis. Moreover, we discovered a specific subclade of plant ubiquitin regulatory X (UBX) domain-containing proteins (PUXs), including PUX3, PUX4, and PXU5, that negatively regulate this process. The three PUX proteins associate with the nucleoskeleton to target the INM, where they physically interact with CDC48, likely directly regulating the activity or substrate specificity of CDC48. Our findings provide direct evidence for the existence of plant INM-associated degradation pathway and uncover a nucleoskeleton-associated regulatory mechanism for it.
HostingRepository	iProX
AnnounceDate	2020-03-11
AnnouncementXML	Submission_2023-03-01_19:26:51.777.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yu Tang
SpeciesList	scientific name: Arabidopsis thaliana; NCBI TaxID: 3702;
ModificationList	N6-biotinyl-L-lysine
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-20 18:52:51	ID requested
1	2020-03-10 21:27:35	announced
⏵ 2	2023-03-01 19:26:52	announced	2023-03-02: Update publication information.

Huang A, Tang Y, Shi X, Jia M, Zhu J, Yan X, Chen H, Gu Y, Proximity labeling proteomics reveals critical regulators for inner nuclear membrane protein degradation in plants. Nat Commun, 11(1):3284(2020) [pubmed]

submitter keyword: Arabidopsis, INMAD, PUX3/4/5, UFD1B/1C, CDC48A/B/C

Yangnan Gu
contact affiliation	Department of Plant & Microbial Biology,University of California, Berkeley
contact email	guyangnan@berkeley.edu
lab head
Yu Tang
contact affiliation	Tsinghua University
contact email	tang414yu@163.com
dataset submitter

iProX dataset URI