PXD015895

PXD015895 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Combining state-of-the-art tandem mass spectrometry with de novo-assisted database peptide sequencing is a viable strategy for the discovery of small signaling peptides at the plant-microbe interface
Description	Small polypeptides (sPEPs) less than 100 amino acids in length are emerging as key signaling molecules that mediate cell-cell communication and biological processes that occur within and between plants, fungi, and bacteria. In this study, we benchmarked the qualitative and quantitative performance of a 10 kDa MWCO filter enrichment strategy optimized specifically for sPEPs using shotgun proteomics with de novo-assisted database searches. We implemented this approach to identify unknown sPEPs from different tissues of Populus X canescens growing with or without Laccaria bicolor ectomycorrhiza. In total, 1660 and 2870 Populus and L. bicolor unique sPEPs were identified, respectively. By means of relative quantification, 129 sPEPs were determined to be significantly more abundant in Populus root with established ectomycorrhizae (ECM) when compared to free-living mycelium of L. bicolor. Several of these peptides mapped to proteins already implicated in biologically relevant associations between plant and fungus.
HostingRepository	MassIVE
AnnounceDate	2020-07-16
AnnouncementXML	Submission_2020-07-16_05:56:21.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Paul Abraham
SpeciesList	scientific name: Populus sp.; NCBI TaxID: 3697;
ModificationList	Carbamidomethyl; Oxidation
Instrument	Q Exactive Plus

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-18 05:35:46	ID requested
⏵ 1	2020-07-16 05:56:21	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: tandem mass spectrometry, liquid chromatography, small peptides, de novo peptide sequencing

submitter keyword: tandem mass spectrometry, liquid chromatography, small peptides, de novo peptide sequencing

Contact List

Paul Abraham
contact affiliation	ORNL
contact email	pav@ornl.gov
lab head
Paul Abraham
contact affiliation	ORNL
contact email	pav@ornl.gov
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000084471/