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PXD015894

PXD015894 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe protein interactomes of AtNHR2A and AtNHR2B unraveled common and specialized functions in plant immunity integrating distinct biological processes
DescriptionAtNHR2A (Arabidopsis thaliana nonhost resistance 2A) and AtNHR2B (Arabidopsis thaliana nonhost resistance 2B) are two proteins required in nonhost resistance, a broad spectrum mechanism of plant immunity that protects plants against pathogens. AtNHR2A and AtNHR2B are localized to the cytoplasm, chloroplasts and other subcellular compartments of unknown identity. The multiple localizations of AtNHR2A and AtNHR2B, suggest that they are highly dynamic and versatile proteins likely participating in multiple biological processes. In spite of their importance, their specific functions have not been elucidated. Thus, to aid in the functional characterization of these proteins and identify the biological processes in which these proteins participate, we used immunoprecipitation coupled with mass spectrometry (IP-MS), to identify the proteins interacting with AtNHR2A and AtNHR2B and generate their interactome network. Further analysis of the AtNHR2A- and AtNHR2B-interacting proteins using published empirical information revealed that AtNHR2A and AtNHR2B function is not limited to plant immunity, highlighting that these proteins are hubs integrating defense outputs with other biological processes.
HostingRepositoryPRIDE
AnnounceDate2020-03-10
AnnouncementXMLSubmission_2020-06-12_02:41:43.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD015894
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterRohana Liyanage
SpeciesList scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationListPhospho; Deamidated; Oxidation; Carbamidomethyl
InstrumentLTQ Orbitrap; Bruker Daltonics amaZon series
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-10-18 03:59:23ID requested
12020-03-10 00:03:20announced
22020-06-12 02:41:44announced2020-06-12: Updated publication reference for PubMed record(s): 32194606.
Publication List
Singh R, Liyanage R, Gupta C, Lay JO, Pereira A, Rojas CM, The Arabidopsis Proteins AtNHR2A and AtNHR2B Are Multi-Functional Proteins Integrating Plant Immunity With Other Biological Processes. Front Plant Sci, 11():232(2020) [pubmed]
Keyword List
submitter keyword: Arabidopsis, Co-Immunoprecipitation, mass spectrometry, affinity purification, plant immunity
Contact List
Clemencia M. Rojas
contact affiliationDepartment of Plant Pathology, University of Arkansas, Fayetteville, AR, USA
contact emailcr022@uark.edu
lab head
Rohana Liyanage
contact affiliationUniversity of Arkansas
contact emailrliyana@uark.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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