PXD015894

PXD015894 is an original dataset announced via ProteomeXchange.

Title	The protein interactomes of AtNHR2A and AtNHR2B unraveled common and specialized functions in plant immunity integrating distinct biological processes
Description	AtNHR2A (Arabidopsis thaliana nonhost resistance 2A) and AtNHR2B (Arabidopsis thaliana nonhost resistance 2B) are two proteins required in nonhost resistance, a broad spectrum mechanism of plant immunity that protects plants against pathogens. AtNHR2A and AtNHR2B are localized to the cytoplasm, chloroplasts and other subcellular compartments of unknown identity. The multiple localizations of AtNHR2A and AtNHR2B, suggest that they are highly dynamic and versatile proteins likely participating in multiple biological processes. In spite of their importance, their specific functions have not been elucidated. Thus, to aid in the functional characterization of these proteins and identify the biological processes in which these proteins participate, we used immunoprecipitation coupled with mass spectrometry (IP-MS), to identify the proteins interacting with AtNHR2A and AtNHR2B and generate their interactome network. Further analysis of the AtNHR2A- and AtNHR2B-interacting proteins using published empirical information revealed that AtNHR2A and AtNHR2B function is not limited to plant immunity, highlighting that these proteins are hubs integrating defense outputs with other biological processes.
HostingRepository	PRIDE
AnnounceDate	2020-03-10
AnnouncementXML	Submission_2020-06-12_02:41:43.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD015894
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Rohana Liyanage
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	Phospho; Deamidated; Oxidation; Carbamidomethyl
Instrument	LTQ Orbitrap; Bruker Daltonics amaZon series

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-18 03:59:23	ID requested
1	2020-03-10 00:03:20	announced
⏵ 2	2020-06-12 02:41:44	announced	2020-06-12: Updated publication reference for PubMed record(s): 32194606.

Singh R, Liyanage R, Gupta C, Lay JO, Pereira A, Rojas CM, The Arabidopsis Proteins AtNHR2A and AtNHR2B Are Multi-Functional Proteins Integrating Plant Immunity With Other Biological Processes. Front Plant Sci, 11():232(2020) [pubmed]

submitter keyword: Arabidopsis, Co-Immunoprecipitation, mass spectrometry, affinity purification, plant immunity

Clemencia M. Rojas
contact affiliation	Department of Plant Pathology, University of Arkansas, Fayetteville, AR, USA
contact email	cr022@uark.edu
lab head
Rohana Liyanage
contact affiliation	University of Arkansas
contact email	rliyana@uark.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/03/PXD015894

PRIDE project URI

• PRIDE
  1. PXD015894
     1. Label: PRIDE project
     2. Name: The protein interactomes of AtNHR2A and AtNHR2B unraveled common and specialized functions in plant immunity integrating distinct biological processes