PXD015889 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Cysteine alkylating methods in shotgun proteomics and their possible effects on methionine residues |
Description | Neatly every proteomic protocol has a Cysteine reduction and alkylation step in it preceding the trypsin digestion. It needs to be done in order to remove the s-s bonds and prevent proteins from folding, thus, provide better access for trypsin. Among various reagents used for alkylation, iodoacetamide (IAM) seems to be the most common. Although, it has been shown that IAM can cause some undesired side reactions [Chernobrovkin et al., 2015; Muller et al., 2017]. In this work, we have compared the effect of four alkylating agents: iodoacetamide (IAM), 4-vinylpyridine (4-VP), chloroacetamide (CAM) and S-Methyl methanethiosulfonate (MMTS). In addition, we tried to check whether post-treatment with DTT (p-red) is reasonable in the case of irreversible alkylation. |
HostingRepository | PRIDE |
AnnounceDate | 2024-02-14 |
AnnouncementXML | Submission_2024-02-14_09:52:23.033.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Irina Ilina |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-10-18 01:46:33 | ID requested | |
⏵ 1 | 2024-02-14 09:52:23 | announced | |
Publication List
10.1016/j.jprot.2020.104022; |
Kuznetsova KG, Levitsky LI, Pyatnitskiy MA, Ilina IY, Bubis JA, Solovyeva EM, Zgoda VG, Gorshkov MV, Moshkovskii SA, Cysteine alkylation methods in shotgun proteomics and their possible effects on methionine residues. J Proteomics, 231():104022(2021) [pubmed] |
Keyword List
submitter keyword: cysteine,proteomics, alkylation, methionine, reduction |
Contact List
Sergei A. Moshkovskii |
contact affiliation | Lab. Med. Proteomics Institute of Biomedical Chemistry Chair of Biochemistry, Medico-Biological Faculty Pirogov Russian National Research Medical University, Moscow, Russia ( lab head ) |
contact email | smosh@mail.ru |
lab head | |
Irina Ilina |
contact affiliation | IBMC |
contact email | irinailina.ibmc@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD015889
- Label: PRIDE project
- Name: Cysteine alkylating methods in shotgun proteomics and their possible effects on methionine residues