PXD015879

PXD015879 is an original dataset announced via ProteomeXchange.

Title	High glucose treatment promotes extracellular matrix proteome remodeling in Müller glial cells
Description	The underlying pathomechanisms in diabetic retinopathy (DR) remains incompletely understood. The aim of this study was to add to the current knowledge about the particular role of retina Müller glial cells (RMG) in the initial processes of DR. Applying a mass spectrometric workflow, we investigated proteome changes of primary porcine RMG under short term diabetic cell culture treatment. We revealed global changes in RMG proteome indicated by a fundamental remodeling of ECM and focal adhesion processes which potentially leads to destabilization of the retinal inner limiting membrane. This is in line with our previous findings of a disrupted ILM in the retinae of a diabetic pig model and suggests an involvement of RMG in this process. Additionally, employing porcine organotypic retinal explant cultures, we demonstrate a specific decrease of RMG-associated SPP1 expression as a result of osmotic challenge induced by high glucose levels. We assume a loss of neuroprotective potential administrated by RMG and subsequent acceleration of neurodegenerative processes in DR.
HostingRepository	PRIDE
AnnounceDate	2021-04-07
AnnouncementXML	Submission_2021-04-06_22:18:18.489.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sandra Sagmeister
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-17 23:39:29	ID requested
1	2021-04-06 02:40:27	announced
⏵ 2	2021-04-06 22:18:18	announced	2021-04-07: Updated project metadata.

Dataset with its publication pending

curator keyword: Biological

submitter keyword: inner limiting membrane (ILM), Diabetic Retinopathy (DR), extracellular matrix (ECM),Müller cells (RMG), hyperglycemia, secreted phosphoprotein 1 (SPP1), neurodegeneration

Stefanie M Hauck
contact affiliation	Research Unit Protein Science, Helmholtz Center Munich, Germany
contact email	hauck@helmholtz-muenchen.de
lab head
Sandra Sagmeister
contact affiliation	LMU München Helmholtz Zentrum München
contact email	sandra.sagmeister@helmholtz-muenchen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/04/PXD015879

PRIDE project URI

• PRIDE
  1. PXD015879
     1. Label: PRIDE project
     2. Name: High glucose treatment promotes extracellular matrix proteome remodeling in Müller glial cells