PXD015878

PXD015878 is an original dataset announced via ProteomeXchange.

Title	LGALS3BP modulates local gyrification in the human brain
Description	Basal radial glial cells (bRGs) are neural progenitors enriched in primates and humans and were proposed to contribute to the expansion of neurons during cortical development in gyrencephalic species. Shortly after their generation, bRGs delaminate towards the outer subventricular zone, where they divide multiple times before differentiation. Thus, the regulation of bRGs generation could be essential for the establishment of correct gyrification within the human cortex. Here, we study the role of LGALS3BP, a secreted protein whose RNA expression is enriched in bRGs. By using cerebral organoids, human fetal tissues and mice, we show that manipulation of LGALS3BP regulated bRG generation. Additionally, individuals with unique de novo variants in LGALS3BP demonstrate abnormal gyrification and thickness at multiple sites over their cortex. Single-cell-RNA-sequencing and proteomics reveal the extracellular matrix involvement in the LGALS3BP mediated mechanisms. We find that LGALS3BP is required for bRGs delamination and influences corticogenesis and gyrification in humans.
HostingRepository	PRIDE
AnnounceDate	2021-10-26
AnnouncementXML	Submission_2021-10-26_07:04:05.530.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pavel Kielkowski
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-17 23:14:40	ID requested
⏵ 1	2021-10-26 07:04:05	announced

Dataset with its publication pending

curator keyword: Biological

submitter keyword: human brain development, human cortical gyrification, evolution, basal radial glial cells, extracellular matrix, LGALS3BP, cerebral organoids, developmental disorders, delamination

Silvia Cappello
contact affiliation	Max Planck Institute of Psychiatry Kraepelinstrasse 2 80804 Munich Germany
contact email	silvia_cappello@psych.mpg.de
lab head
Pavel Kielkowski
contact affiliation	Technical University of Munich, Chair of Organic Chemistry II, Lichtenberstrasse 4, 85764, Garching
contact email	pavel.kielkowski@tum.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/10/PXD015878

PRIDE project URI

• PRIDE
  1. PXD015878
     1. Label: PRIDE project
     2. Name: LGALS3BP modulates local gyrification in the human brain