PXD015877

PXD015877 is an original dataset announced via ProteomeXchange.

Title	The length of a ubiquitin chain is a general determinant for selective recognition by ubiquitin-binding proteins.
Description	The attachment of differently linked ubiquitin (Ub) chains of varying length to proteins is a prevalent posttranslational modification in eukaryotic cells. The fate of a modified protein is determined by Ub-binding proteins (UBPs) that interact with Ub chains in a linkage-selective manner. Therefore, proteome-wide interaction studies using differently linked Ub chains have become a focus of research activities. However, the impact and functional consequences of chain length on the binding selectivity of UBPs remain mostly elusive, due to a lack of available tools and sufficient amounts of pure, length-defined Ub chains. Here we generated linkage- and length-defined Ub chains using click-chemistry and gel-free fractionation and employed such defined polymers in affinity-based enrichment assays to identify length- and linkage-selective interactors on a proteome-wide scale. For the first time, this revealed that the length of a Ub chain has generally a major impact on its ability to be selectively recognized by UBPs.
HostingRepository	PRIDE
AnnounceDate	2020-04-20
AnnouncementXML	Submission_2020-04-19_23:42:43.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Florian Stengel
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-17 23:12:40	ID requested
⏵ 1	2020-04-19 23:42:44	announced

Dataset with its publication pending

curator keyword: Biological

submitter keyword: ubiquitin, chain-length, Ub-binding proteins, posttranslational modification

Florian Stengel
contact affiliation	Department of Biology - Cellular Proteostasis and Mass Spectrometry, University of Konstanz
contact email	florian.stengel@uni-konstanz.de
lab head
Florian Stengel
contact affiliation	Universität Konstanz
contact email	florian.stengel@uni-konstanz.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/04/PXD015877

PRIDE project URI

• PRIDE
  1. PXD015877
     1. Label: PRIDE project
     2. Name: The length of a ubiquitin chain is a general determinant for selective recognition by ubiquitin-binding proteins.