PXD015856 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Protein partners identification in Pyrococcus abyssi by nanoLC-MSMS |
Description | Recombinant aRNase J from P. abyssi, with C-terminal (His)6-tag (aRNase J-His), was produced, purified and used as baits for AP-MS experiments. In order to discriminate specific protein interaction from column background, we performed mock AP-MS analyses with P. abyssi cell extracts in absence of protein bait. In addition, to determine if some interactions are mediated through RNA or DNA, we implemented a nuclease treatment after incubating the cell extract with the bait. The co-purified partners were identified by bottom-up proteomic techniques coupled with mass spectrometry. |
HostingRepository | PRIDE |
AnnounceDate | 2020-01-21 |
AnnouncementXML | Submission_2020-01-21_01:17:50.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Lydie Correia |
SpeciesList | scientific name: Pyrococcus abyssi; NCBI TaxID: 29292; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-10-16 05:46:15 | ID requested | |
⏵ 1 | 2020-01-21 01:17:51 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Pyrococcus abyssi, pull-down, nanoLC-MSMS |
Contact List
Lydie Oliveira Correia |
contact affiliation | INRA MICALIS PAPPSO |
contact email | lydie.oliveira-correia@inra.fr |
lab head | |
Lydie Correia |
contact affiliation | INRA PAPPSO |
contact email | lydie.oliveira-correia@inra.fr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD015856
- Label: PRIDE project
- Name: Protein partners identification in Pyrococcus abyssi by nanoLC-MSMS