PXD015848

PXD015848 is an original dataset announced via ProteomeXchange.

Title	Upstream ORF-encoded ASDURF is a novel prefoldin-like subunit of the PAQosome
Description	The PAQosome is an eleven-subunit chaperone involved in the biogenesis of several human protein complexes. We show that ASDURF, a recently discovered upstream open reading frame (uORF) in the 5’ UTR of ASNSD1 mRNA, encodes the twelfth subunit of the PAQosome. ASDURF, displays significant structural homology to beta-prefoldins and assembles with the five known subunits of the prefoldin-like module of the PAQosome to form a heterohexameric prefoldin-like complex. A model of the PAQosome prefoldin-like module is presented. The data presented here provides an example of ana eukaryotic uORF-encoded polypeptide whose function is not limited to cis-acting translational regulation of downstream coding sequence and highlights the importance of including alternative ORF products in proteomic studies.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:03:27.296.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christian Poitras
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-16 02:35:10	ID requested
1	2019-11-25 09:57:58	announced
⏵ 2	2024-10-22 04:03:29	announced	2024-10-22: Updated project metadata.

10.1021/acs.jproteome.9b00599;

Cloutier P, Poitras C, Faubert D, Bouchard A, Blanchette M, Gauthier MS, Coulombe B, Upstream ORF-Encoded ASDURF Is a Novel Prefoldin-like Subunit of the PAQosome. J Proteome Res, 19(1):18-27(2020) [pubmed]

submitter keyword: AP-MS, protein complex assembly, uORF, PAQosome, small ORF encoded peptide, chaperone,ASDURF, BioID, R2TP/PFDL, prefoldin

Benoit Coulombe
contact affiliation	Translational Proteomics Laboratory, Institut de recherches cliniques de Montréal (IRCM), Québec, Montréal, Canada. Département de biochimie et médecine moléculaire, Université de Montréal, Québec, Montréal, Canada.
contact email	benoit.coulombe@ircm.qc.ca
lab head
Christian Poitras
contact affiliation	Laboratory of Gene Transcription and Proteomics Discovery Platform, Institut de recherches cliniques de Montréal, 110 avenue des Pins Ouest, Montréal, QC H2W 1R7, Canada
contact email	christian.poitras@ircm.qc.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD015848

PRIDE project URI

• PRIDE
  1. PXD015848
     1. Label: PRIDE project
     2. Name: Upstream ORF-encoded ASDURF is a novel prefoldin-like subunit of the PAQosome