PXD015847

PXD015847 is an original dataset announced via ProteomeXchange.

Title	A proximity-labeling proteomic approach to investigate invadopodia molecular landscape in breast cancer cells
Description	Invadopodia constitute a specialized machinery required for invasive tumor cells to travel through basement membranes and the interstitial matrix, and thus represent a key feature of metastatic cells. Therefore a better understanding of invadopodia biology and characteristics might permit the identification of markers of metastatic potential. Substantial efforts have been made to characterize invadopodia molecular composition, dynamics or triggering stimuli. However, the full molecular identity of these structures is still missing due to the fact that the isolation and purification of these structures has been challenging. To fill this gap, we developed a non-hypothesis driven proteomic approach based on the innovative BioID proximity biotinylation approach using the invadopodia-specific protein Tks5/FISH (its long form, Tks5) fused to the biotin ligase BirA as bait. As control, we also generated cells expressing the short form of Tks5 (Tks5) unable to localize to invadopodia. After validation of our cell models, Tks5 close neighbors were identified by label-free mass spectrometry after pulldown of biotinylated proteins. Known invadopodia components were identified revealing the pertinence of our strategy. Furthermore, this strategy allowed us to identify novel Tks5 in cellulo partners that appear as potential new invadopodia components/regulators and potential markers of metastasis.
HostingRepository	PRIDE
AnnounceDate	2020-04-24
AnnouncementXML	Submission_2020-07-15_00:13:18.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	AUDEBERT Stephane
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-16 02:34:55	ID requested
1	2020-04-24 02:11:27	announced
⏵ 2	2020-07-15 00:13:19	announced	2020-07-15: Updated publication reference for PubMed record(s): 32321993.

Thuault S, Mamelonet C, Salameh J, Ostacolo K, Chanez B, Sala, ü, n D, Baudelet E, Audebert S, Camoin L, Badache A, A proximity-labeling proteomic approach to investigate invadopodia molecular landscape in breast cancer cells. Sci Rep, 10(1):6787(2020) [pubmed]

submitter keyword: Tks5/SH3PXD2A, actin cytoskeleton, cancer invasion

Camoin Luc
contact affiliation	Centre de Recherche en Cancérologie de Marseille, CRCM Marseille Proteomique Inserm UMR1068, CNRS UMR7258, Aix Marseille Université U105,z Institut Paoli Calmettes 27 Boulevard Leï Roure CS30059 13273 Marseille Cedex 09 France
contact email	Luc.camoin@inserm.fr
lab head
AUDEBERT Stephane
contact affiliation	Pharmacology department
contact email	stephane.audebert@inserm.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/04/PXD015847

PRIDE project URI

• PRIDE
  1. PXD015847
     1. Label: PRIDE project
     2. Name: A proximity-labeling proteomic approach to investigate invadopodia molecular landscape in breast cancer cells