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PXD015830

PXD015830 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePPARgamma ubiquitination sites in HEK293T cells
DescriptionPeroxisome proliferator–activated receptor γ (PPARγ) is the central regulator of adipogenesis, and its dysregulation is linked to obesity and metabolic diseases. Identification of the factors that regulate PPARγ expression and activity is therefore crucial for combating obesity. Aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor with a known role in xenobiotic detoxification. Recent studies have suggested that AhR also plays essential roles in energy metabolism. However, the detailed mechanisms remain unclear. We previously reported that experiments with adipocyte-specific Cullin 4b (Cul4b)-knockout mice showed that CUL4B suppresses adipogenesis by targeting PPARγ. Here, using immunoprecipitation, ubiquitination, real-time PCR and Gst-pulldown assays, we report that AhR functions as the substrate receptor in CUL4B–RING E3 ubiquitin ligase (CRL4B) complex and is required for recruiting PPARγ. AhR overexpression reduced PPARγ stability and suppressed adipocyte differentiation, and AhR knockdown stimulated adipocyte differentiation in 3T3-L1 cells. Furthermore, we found that two lysine sites on residues 268 and 293 in PPARγ are targeted for CRL4B-mediated ubiquitination, indicating cross-talk between acetylation and ubiquitination. Our findings establish a critical role of AhR in regulating PPARγ stability and suggest that the AhR–PPARγ interaction may represent a potential therapeutic target for managing metabolic diseases arising from PPARγ dysfunction.
HostingRepositoryPRIDE
AnnounceDate2019-11-01
AnnouncementXMLSubmission_2019-11-01_05:00:11.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterHao Dou
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListubiquitinylated lysine
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-10-15 06:00:31ID requested
12019-11-01 05:00:12announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: PPARgamma LC-MS/MS HEK293T
Contact List
Yaoqin Gong
contact affiliationInstitute of Molecular Medicine and Genetics,Shandong University, PR China
contact emailyxg8@sdu.edu.cn
lab head
Hao Dou
contact affiliationAssociate Professor
contact emaildouhao@sdu.edu.cn
dataset submitter
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Dataset FTP location
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