PXD015827

PXD015827 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Starvation-specific NSPs in Leishmania mexicana parasites by quantitative proteomics
Description	Using a combination of BONCAT technique and iTRAQ 3-plex quantitative proteomics MS, we profiled the de novo protein synthesis in the starving L. mexicana parasites in a temporally resolved manner. This approach uniquely integrates the high specificity of the BONCAT technique for the NSPs with the high sensitivity and multiplexed quantitation capability of the iTRAQ quantitative proteomics MS. Proof-of-concept experiments identified over 250 starvation-specific NSPs in the parasite and quantified the relative changes in abundance of these proteins as a function of duration of starvation.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:08:35.239.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kalesh Karunakaran
SpeciesList	scientific name: Trypanosoma brucei; NCBI TaxID: 5691;
ModificationList	iTRAQ4plex-116 reporter+balance reagent acylated residue
Instrument	TripleTOF 5600

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-15 01:27:28	ID requested
1	2019-12-04 00:42:23	announced
2	2020-01-14 23:51:31	announced	2020-01-15: Updated publication reference for PubMed record(s): 31856154.
⏵ 3	2024-10-22 04:08:37	announced	2024-10-22: Updated project metadata.

Publication List

10.1371/journal.pntd.0007651;
Kalesh K, Denny PW, A BONCAT-iTRAQ method enables temporally resolved quantitative profiling of newly synthesised proteins in Leishmania mexicana parasites during starvation. PLoS Negl Trop Dis, 13(12):e0007651(2019) [pubmed]

10.1371/journal.pntd.0007651;

Kalesh K, Denny PW, A BONCAT-iTRAQ method enables temporally resolved quantitative profiling of newly synthesised proteins in Leishmania mexicana parasites during starvation. PLoS Negl Trop Dis, 13(12):e0007651(2019) [pubmed]

Keyword List

submitter keyword: Newly synthesised proteins, starvation,BONCAT, Leishmania, iTRAQ

submitter keyword: Newly synthesised proteins, starvation,BONCAT, Leishmania, iTRAQ

Contact List

Dr Kalesh Karunakaran
contact affiliation	Department of Chemistry, Durham University, Durham, DH1 3LE, UK
contact email	kalesh.karunakaran@durham.ac.uk
lab head
Kalesh Karunakaran
contact affiliation	Durham University
contact email	kalesh.karunakaran@durham.ac.uk
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/12/PXD015827
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/12/PXD015827

PRIDE project URI

Repository Record List

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