PXD015823 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The RNA-binding protein ATXN2 controls cellular processes during megakaryopoiesis |
Description | Megakaryocytes accumulate mRNA during their maturation, which is required for the correct spatio-temporal production of cytoskeletal proteins, membranes and platelet-specific granules, and for the subsequent shedding of thousands of platelets per cell. Gene expression profiling identified the RNA binding protein ATAXIN2 (ATXN2) as a putative novel regulator of megakaryopoiesis. ATXN2 expression is high in CD34+/CD41+ megakaryoblasts and sharply decreases upon maturation to megakaryocytes. ATXN2 associates with DDX6 suggesting that it may mediate repression of mRNA translation during early megakaryopoiesis. Comparative transcriptome and proteome analysis on megakaryoid cells (MEG-01) with differential ATXN2 expression identified ATXN2 dependent gene expression of mRNA and protein involved in processes linked to coagulation. Mice deficient for Atxn2 did not display differences in bleeding times, but expression of key surface receptors on platelets, such as ITGB3 (carries the CD61 antigen) and CD31 (PECAM1), were deregulated and platelet aggregation was reduced upon specific triggers. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:05:29.863.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Arie Hoogendijk |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-10-15 01:21:40 | ID requested | |
1 | 2020-05-26 15:06:02 | announced | |
⏵ 2 | 2024-10-22 05:05:32 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.3390/ijms21030967; |
Hansen M, Zeddies S, Meinders M, di Summa F, van Alphen FPJ, Hoogendijk AJ, Moore KS, Halbach M, Guti, é, rrez L, van den Biggelaar M, Thijssen-Timmer DC, Auburger GWJ, van den Akker E, von Lindern M, Rollmann E, The RNA-Binding Protein ATXN2 is Expressed during Megakaryopoiesis and May Control Timing of Gene Expression. Int J Mol Sci, 21(3):(2020) [pubmed] |
Keyword List
submitter keyword: LC-MS, megakaryopoiesis |
Contact List
M. van den Biggelaar, PhD |
contact affiliation | Department of Molecular and Cellular Hemostasis Plasma Proteins, Sanquin Research, Amsterdam, The Netherlands |
contact email | m.vandenbiggelaar@sanquin.nl |
lab head | |
Arie Hoogendijk |
contact affiliation | Sanquin Research, Amsterdam |
contact email | a.hoogendijk@sanquin.nl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD015823 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD015823
- Label: PRIDE project
- Name: The RNA-binding protein ATXN2 controls cellular processes during megakaryopoiesis