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PXD015823

PXD015823 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe RNA-binding protein ATXN2 controls cellular processes during megakaryopoiesis
DescriptionMegakaryocytes accumulate mRNA during their maturation, which is required for the correct spatio-temporal production of cytoskeletal proteins, membranes and platelet-specific granules, and for the subsequent shedding of thousands of platelets per cell. Gene expression profiling identified the RNA binding protein ATAXIN2 (ATXN2) as a putative novel regulator of megakaryopoiesis. ATXN2 expression is high in CD34+/CD41+ megakaryoblasts and sharply decreases upon maturation to megakaryocytes. ATXN2 associates with DDX6 suggesting that it may mediate repression of mRNA translation during early megakaryopoiesis. Comparative transcriptome and proteome analysis on megakaryoid cells (MEG-01) with differential ATXN2 expression identified ATXN2 dependent gene expression of mRNA and protein involved in processes linked to coagulation. Mice deficient for Atxn2 did not display differences in bleeding times, but expression of key surface receptors on platelets, such as ITGB3 (carries the CD61 antigen) and CD31 (PECAM1), were deregulated and platelet aggregation was reduced upon specific triggers.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:05:29.863.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterArie Hoogendijk
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-10-15 01:21:40ID requested
12020-05-26 15:06:02announced
22024-10-22 05:05:32announced2024-10-22: Updated project metadata.
Publication List
10.3390/ijms21030967;
Hansen M, Zeddies S, Meinders M, di Summa F, van Alphen FPJ, Hoogendijk AJ, Moore KS, Halbach M, Guti, é, rrez L, van den Biggelaar M, Thijssen-Timmer DC, Auburger GWJ, van den Akker E, von Lindern M, Rollmann E, The RNA-Binding Protein ATXN2 is Expressed during Megakaryopoiesis and May Control Timing of Gene Expression. Int J Mol Sci, 21(3):(2020) [pubmed]
Keyword List
submitter keyword: LC-MS, megakaryopoiesis
Contact List
M. van den Biggelaar, PhD
contact affiliationDepartment of Molecular and Cellular Hemostasis Plasma Proteins, Sanquin Research, Amsterdam, The Netherlands
contact emailm.vandenbiggelaar@sanquin.nl
lab head
Arie Hoogendijk
contact affiliationSanquin Research, Amsterdam
contact emaila.hoogendijk@sanquin.nl
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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