PXD015820

PXD015820 is an original dataset announced via ProteomeXchange.

Title	Cell-specific non-canonical amino acid labelling identifies changes in the hippocampal de novo proteome during memory formation
Description	The formation of spatial long-term memory (LTM) requires the de novo synthesis of distinct sets of proteins; however, a non-biased examination of the de novo proteome in this process is lacking. Here, we generated a novel mouse strain, which enables cell-type-specific labelling of newly synthesised proteins with non-canonical amino acids (NCAAs) by genetically restricting the expression of the mutant tRNA synthetase, NLL-MetRS. Combining this labelling technique with an accelerated version of the active place avoidance task, and bio-orthogonal non-canonical amino acid tagging (BONCAT) followed by SWATH quantitative mass spectrometry, we identified 156 proteins that were altered in synthesis in hippocampal neurons during spatial memory formation. In addition to observing increased synthesis of proteins important in known memory-related processes, such as glutamate receptor recycling, we also identified alter synthesised of proteins associated with mRNA splicing and stability as a potential mechanism involved in spatial LTM formation.
HostingRepository	PRIDE
AnnounceDate	2020-05-26
AnnouncementXML	Submission_2020-05-26_15:05:47.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Harrison Evans
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized amino-terminal residue; iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-15 01:21:14	ID requested
⏵ 1	2020-05-26 15:05:48	announced

Evans HT, Bodea LG, G, ö, tz J, Cell-specific non-canonical amino acid labelling identifies changes in the de novo proteome during memory formation. Elife, 9():(2020) [pubmed]

submitter keyword: Active place avoidance, azidohomoalanine, azidonorleucine, long-term memory, mRNA splicing, non-canonical amino acid labelling, protein synthesis, SWATH

Jurgen Gotz
contact affiliation	Queensland Brain Institute University of Queensland
contact email	j.goetz@uq.edu.au
lab head
Harrison Evans
contact affiliation	The University of Queensland
contact email	h.evans@uq.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD015820
     1. Label: PRIDE project
     2. Name: Cell-specific non-canonical amino acid labelling identifies changes in the hippocampal de novo proteome during memory formation