PXD015809
PXD015809 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | First Global Proteomic Analysis of Lysine Malonylation in Toxoplasma gondii |
| Description | Lysine malonylation (Kmal) is a new posttranslational modification (PTM), which has been reported in several prokaryotic and eukaryotic species. Although Kmal can regulate many and diverse biological processes in various organisms, knowledge about this important PTM in the apicomplexan parasite Toxoplasma gondii is limited. In this study, we performed the first global profiling of malonylated proteins in T. gondii tachyzoites using affinity enrichment and LC-MS/MS analysis. Three experiments performed in tandem revealed 331, 367, 373 Kmal sites on 227, 252, 241 malonylated proteins, respectively. Computational analysis showed the identified malonylated proteins to be localized in various subcellular compartments and involved in many cellular functions, particularly mitochondrial function and oxidation of fatty acids. Additionally, two conserved Kmal motifs with a strong bias for cysteine were detected. Taken together, these findings provide the first report of Kmal profile in T. gondii and should be an important resource for studying the physiological roles of Kmal in this parasite. |
| HostingRepository | PRIDE |
| AnnounceDate | 2024-10-22 |
| AnnouncementXML | Submission_2024-10-22_05:06:26.470.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Lan-Be Nie |
| SpeciesList | scientific name: Toxoplasma gondii; NCBI TaxID: 5811; |
| ModificationList | N6-malonyl-L-lysine |
| Instrument | Q Exactive Plus |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2019-10-14 02:05:08 | ID requested | |
| 1 | 2020-05-27 02:56:26 | announced | |
| ⏵ 2 | 2024-10-22 05:06:27 | announced | 2024-10-22: Updated project metadata. |
Publication List
| Nie LB, Liang QL, Du R, Elsheikha HM, Han NJ, Li FC, Zhu XQ, . Front Microbiol, 11():776(2020) [pubmed] |
| 10.3389/fmicb.2020.00776; |
Keyword List
| submitter keyword: Post-translational |
| modifications,Toxoplasma gondii,malonylome,lysine malonylation |
Contact List
| Xing-Quan Zhu | |
|---|---|
| contact affiliation | State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province 730046, PR China |
| contact email | xingquanzhu1@hotmail.com |
| lab head | |
| Lan-Be Nie | |
| contact affiliation | State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou |
| contact email | nielanbi188@163.com |
| dataset submitter | |
Full Dataset Link List
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD015809 |
| PRIDE project URI |
Repository Record List
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