PXD015808

PXD015808 is an original dataset announced via ProteomeXchange.

Title	TMT quantitative proteomics of a) protrusions and cells bodies of a panel of cell lines, b) protrusions and cells bodies of MDA-MB231 cells collected after 1 h, 2 h, 4 h, 8 h post- induction of protrusions, c) non-transfected (nt) or LARP6 siRNAs MDA-MB231 cells grown on either close or open collagen coated transwells and d) protrusions and cells bodies of a panel of breast cell lines.
Description	Translation of Ribosomal Protein coding mRNAs (RP-mRNAs) constitutes a key step in regulation of ribosome biogenesis in human cells, but the exact mechanisms which modulate RP-mRNAs translation under various cellular and environmental conditions remain poorly understood. Here we show that the subcellular localisation of RP-mRNAs acts as a key regulator of their translation in mesenchymal-like migratory cells. As cells invade into their surroundings, RP-mRNAs localise to the actin-rich protrusions at the front of the cells. This localisation is mediated by La related protein-6 (LARP6), an RNA Binding Protein (RBP) that is enriched in protrusions. Importantly, translation initiation and elongation factors are also enriched in protrusions. LARP6 dependent localisation of RP-mRNAs enhances their translation, leading to up-regulation of ribosome biogenesis and increased overall protein synthesis. In breast carcinomas, enhanced expression of LARP6 is associated with Epithelial to Mesenchymal Transition (EMT), and can be therapeutically targeted by a small molecule inhibitor which interferes with LARP6 RNA binding. These findings reveal an RNA localisation based post-transcriptional mechanism that governs ribosome biogenesis in migratory cells, and implicate a role for this process in cancer progression downstream of EMT.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:26:36.418.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Maria Dermit Salazar
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-14 02:04:56	ID requested
1	2021-09-08 14:00:43	announced
⏵ 2	2024-10-22 05:26:42	announced	2024-10-22: Updated project metadata.

10.1016/j.xpro.2021.100462;

Dermit M, Mardakheh FK, Purification and quantitative proteomic analysis of cell bodies and protrusions. STAR Protoc, 2(2):100462(2021) [pubmed]

submitter keyword: Human, malignant and non-malignant cell lines, LC-MS/MS

Faraz K. Mardakheh
contact affiliation	Centre for Cancer Cell and Molecular Biology, Barts Cancer Institute, Queen Mary University of London, Charterhouse square, London EC1M 6BQ, United Kingdom.
contact email	f.mardakheh@qmul.ac.uk
lab head
Maria Dermit Salazar
contact affiliation	qmul
contact email	mariaprideproteomics@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD015808

PRIDE project URI

• PRIDE
  1. PXD015808
     1. Label: PRIDE project
     2. Name: TMT quantitative proteomics of a) protrusions and cells bodies of a panel of cell lines, b) protrusions and cells bodies of MDA-MB231 cells collected after 1 h, 2 h, 4 h, 8 h post- induction of protrusions, c) non-transfected (nt) or LARP6 siRNAs MDA-MB231 cells grown on either close or open collagen coated transwells and d) protrusions and cells bodies of a panel of breast cell lines.