PXD015786

PXD015786 is an original dataset announced via ProteomeXchange.

Title	Herpesvirus infection reduces Pol II occupancy of host promoters but spares viral promoters
Description	In mammalian cells, widespread acceleration of cytoplasmic mRNA degradation is linked to impaired RNA polymerase II (Pol II) transcription. This mRNA decay-induced transcriptional repression occurs during infection with gammaherpesviruses including Kaposi’s sarcoma associated herpesvirus (KSHV) and murine gammaherpesvirus 68 (MHV68), which encode an mRNA endonuclease that initiates widespread RNA decay. Here, we show that MHV68-induced mRNA decay leads to a genome-wide reduction of Pol II occupancy at mammalian promoters. Viral genes, despite the fact that they require Pol II for transcription, escape this transcriptional repression. Protection is not governed by viral promoter sequences; instead, location on the viral genome is both necessary and sufficient to escape the transcriptional repression effects of mRNA decay. We hypothesize that the ability to escape from transcriptional repression is linked to the localization of viral DNA in replication compartments, providing a means for these viruses to counteract decay-induced viral transcript loss.
HostingRepository	PRIDE
AnnounceDate	2019-12-10
AnnouncementXML	Submission_2020-02-17_21:08:06.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joel Federspiel
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-11 01:17:27	ID requested
1	2019-12-10 06:27:59	announced
⏵ 2	2020-02-17 21:08:08	announced	2020-02-18: Updated publication reference for PubMed record(s): 32032393.

Hartenian E, Gilbertson S, Federspiel JD, Cristea IM, Glaunsinger BA, RNA decay during gammaherpesvirus infection reduces RNA polymerase II occupancy of host promoters but spares viral promoters. PLoS Pathog, 16(2):e1008269(2020) [pubmed]

submitter keyword: MHV68, TMT, Herpesvirus, Infection, RNA Pol II

Ileana M Cristea
contact affiliation	Department of Molecular Biology, Princeton University, USA
contact email	icristea@princeton.edu
lab head
Joel Federspiel
contact affiliation	Princeton University
contact email	joel.federspiel@princeton.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/12/PXD015786

PRIDE project URI

• PRIDE
  1. PXD015786
     1. Label: PRIDE project
     2. Name: Herpesvirus infection reduces Pol II occupancy of host promoters but spares viral promoters