PXD015770

PXD015770 is an original dataset announced via ProteomeXchange.

Title	Recombinant glycosyl hydrolase expression coupled with adaptation resembling sucrose-induction enable cellobiose-to-ethanol conversion and growth in Zymomonas mobilis
Description	We engineered Z. mobilis ZM4 to express a recombinant glycosyl hydrolase (GH) from Caulobacter crescentus and subjected it to an adaptation in cellobiose medium. Growth on cellobiose was achieved after a prolonged lag phase in cellobiose medium that induced changes in gene expression and cell composition, including increased expression and secretion of GH. These changes were found to be reversible, i.e. not a genetic adaptation, but could be preserved upon transfer to fresh cellobiose medium. After adaptation to cellobiose, our GH expressing strain was able to convert about 50% of cellobiose to glucose within 24 h and use it for growth and ethanol production. Alternatively, adaptation by growth in sucrose medium also enabled immediate growth of Z. mobilis GH3 on cellobiose. Proteomics analysis of cellobiose- and sucrose-adapted strains revealed upregulation of secretion-, transport-, and outer membrane-related proteins, which may aid secretion of GH into the medium. Changes in the cell envelope may additionally enable entry of cellobiose into the cell periplasm or surface display of GHs.
HostingRepository	PRIDE
AnnounceDate	2020-07-29
AnnouncementXML	Submission_2020-07-28_23:04:36.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Evgenia Shishkova
SpeciesList	scientific name: Zymomonas mobilis; NCBI TaxID: 542;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-10 01:34:40	ID requested
⏵ 1	2020-07-28 23:04:36	announced

Dataset with its publication pending

submitter keyword: Cellobiose utilization, Glycosyl hydrolase, Z. mobilis ZM4, Serial transfer, Oligosaccharide conversion, Biofuels

Joshua J Coon
contact affiliation	Professor of Biomolecular Chemistry and Chemistry at University of Wisconsin, Madison, USA
contact email	jcoon@chem.wisc.edu
lab head
Evgenia Shishkova
contact affiliation	University of Wisconsin - Madison
contact email	shishkova@wisc.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/07/PXD015770

PRIDE project URI

• PRIDE
  1. PXD015770
     1. Label: PRIDE project
     2. Name: Recombinant glycosyl hydrolase expression coupled with adaptation resembling sucrose-induction enable cellobiose-to-ethanol conversion and growth in Zymomonas mobilis