PXD015757

PXD015757 is an original dataset announced via ProteomeXchange.

Title	Engineered fibre enables targeted activation of butyrate-producing microbiota in the distal gut
Description	Beneficial modulation of the gut microbiome has high-impact implications not only in humans, but also in livestock that sustain our current societal needs. In this context, we have engineered an acetylated galactoglucomannan (AcGGM) fibre from spruce trees to match unique enzymatic capabilities of Roseburia and Faecalibacterium species, both renowned butyrate-producing gut commensals. The accuracy of AcGGM was tested in an applied pig feeding trial, which resolved 355 metagenome-assembled genomes together with quantitative metaproteomes. In AcGGM-fed pigs, both target populations differentially expressed AcGGM-specific polysaccharide utilization loci, including novel, mannan-specific esterases that are critical to its deconstruction. We additionally observed a “butterfly effect”, whereby numerous metabolic changes and interdependent cross-feeding pathways were detected in neighboring non-mannolytic populations that produce short-chain fatty acids. Our findings show that intricate structural features and acetylation patterns of dietary fibre can be customized to specific bacterial populations, with the possibility to create greater modulatory effects at large.
HostingRepository	PRIDE
AnnounceDate	2020-09-28
AnnouncementXML	Submission_2020-12-04_01:08:48.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Magnus Arntzen
SpeciesList	scientific name: Coprococcus eutactus; NCBI TaxID: 33043; scientific name: Faecalibacterium prausnitzii; NCBI TaxID: 853; scientific name: Catenibacterium; NCBI TaxID: 135858; scientific name: Butyrivibrio fibrisolvens; NCBI TaxID: 831; scientific name: Roseburia; NCBI TaxID: 841; scientific name: [Ruminococcus] gnavus; NCBI TaxID: 33038; scientific name: Dialister; NCBI TaxID: 39948;
ModificationList	2-pyrrolidone-5-carboxylic acid (Gln); monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-09 05:32:27	ID requested
1	2020-09-28 06:28:30	announced
⏵ 2	2020-12-04 01:08:49	announced	2020-12-04: Updated publication reference for PubMed record(s): 33188211.

Michalak L, Gaby JC, Lagos L, La Rosa SL, Hvidsten TR, T, é, tard-Jones C, Willats WGT, Terrapon N, Lombard V, Henrissat B, Dr, ö, ge J, Arntzen M Ø, Hagen LH, Ø, verland M, Pope PB, Westereng B, Microbiota-directed fibre activates both targeted and secondary metabolic shifts in the distal gut. Nat Commun, 11(1):5773(2020) [pubmed]

submitter keyword: Metaproteomics, LC-MS/MS, porcine colon microbiome

Bjørge Westereng
contact affiliation	Faculty of Chemistry, Biotechnology and Food Science Norwegian University of Life Sciences Post Office Box 5003 1432, Ås Norway
contact email	bjorge.westereng@nmbu.no
lab head
Magnus Arntzen
contact affiliation	Norwegian University of Life Sciences
contact email	magnus.arntzen@nmbu.no
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD015757
     1. Label: PRIDE project
     2. Name: Engineered fibre enables targeted activation of butyrate-producing microbiota in the distal gut