PXD015741

PXD015741 is an original dataset announced via ProteomeXchange.

Title	Exposure to the Florida red tide dinoflagellate, Karenia brevis, and its associated brevetoxins induces ecophysiological and proteomic alterations in Porites astreoides
Description	As reef-building corals are increasingly being exposed to persistent threats that operate on both regional and global scales there is a pressing need to better understand the complex processes that diminish coral populations. This study investigated the impacts of the Florida Red Tide dinoflagellate Karenia brevis and associated brevetoxins on selected facets of coral biology using Porites astreoides as a model system. When provided with choice assays, P. astreoides larvae were shown to actively avoid seawater containing red tide or purified brevetoxins. However, forced exposure to similar treatments induced time-dependent physiological and behavioral changes that were captured by PAM fluorometry and settlement and survival assays, respectively. Adult fragments of P. astreoides exposed to red tide or associated brevetoxins displayed signs of proteomic alterations that were characterized by use of an iTRAQ-based quantitative proteomic analysis. The novel use of this technique on P. astreoides demonstrated that protein expression was highly contingent upon biological versus chemical treatment and that several broad pathways associated with cell stress were affected including redox homeostasis, protein folding, energy metabolism, and reactive oxygen species (ROS) production. The results herein provide new insight into the ecology, behavior and sublethal stress of reef-building corals in response to K. brevis exposure and underscore the importance of recognizing the potential of red tide to act as a regional stressor to these important foundation species.
HostingRepository	MassIVE
AnnounceDate	2022-09-19
AnnouncementXML	Submission_2022-09-19_10:38:02.451.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jin Koh
SpeciesList	scientific name: Karenia brevis; NCBI TaxID: 156230; scientific name: Porites astreoides; NCBI TaxID: 104758;
ModificationList	iTRAQ8plex
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-08 07:04:35	ID requested
⏵ 1	2022-09-19 10:38:03	announced

Reynolds DA, Yoo MJ, Dixson DL, Ross C, Exposure to the Florida red tide dinoflagellate, Karenia brevis, and its associated brevetoxins induces ecophysiological and proteomic alterations in Porites astreoides. PLoS One, 15(2):e0228414(2020) [pubmed]

submitter keyword: iTRAQ, Porites astreoides, Karenia brevis, brevetoxins, proteomics

Cliff Ross
contact affiliation	University of North Florida
contact email	cliff.ross@unf.edu
lab head
Jin Koh
contact affiliation	University of Florida
contact email	jinkoh@ufl.edu
dataset submitter

MassIVE dataset URI

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