PXD015733
PXD015733 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Quantitative In Vivo Proteomics of Metformin Response in Liver Reveals AMPK-Dependent and -Independent Signaling Networks |
Description | Metformin is the front-line treatment for type 2 diabetes worldwide. It acts via effects on glucose and lipid metabolism in metabolic tissues, leading to enhanced insulin sensitivity. Despite significant effort, the molecular basis for metformin response remains poorly understood, with a limited number of specific biochemical pathways studied to date. To broaden our understanding of hepatic metformin response, we combine phospho-protein enrichment in tissue from genetically engineered mice with a quantitative proteomics platform to enable the discovery and quantification of basophilic kinase substrates in-vivo. We define proteins that binding to 14-3-3 are acutely regulated by metformin treatment and/or loss of the serine/threonine kinase, LKB1. Inducible binding of 250 proteins following metformin treatment is observed, 44% LKB1-dependent. Beyond AMPK, metformin activates Protein Kinase D and MAPKAPK2 in an LKB1-independent manner, revealing additional kinases that may mediate aspects of metformin response. Deeper analysis uncovered substrates of AMPK in endocytosis and calcium homeostasis. |
HostingRepository | MassIVE |
AnnounceDate | 2020-01-08 |
AnnouncementXML | Submission_2020-01-08_18:28:00.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Salvador Martínez de Bartolomé |
SpeciesList | scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090; |
ModificationList | Carbamidomethyl |
Instrument | LTQ Orbitrap Elite; LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2019-10-07 15:23:28 | ID requested | |
⏵ 1 | 2020-01-08 18:28:01 | announced |
Publication List
Stein BD, Calzolari D, Hellberg K, Hu YS, He L, Hung CM, Toyama EQ, Ross DS, Lillemeier BF, Cantley LC, Yates JR, Shaw RJ, Vivo Proteomics of Metformin Response in Liver Reveals AMPK-Dependent and -Independent Signaling Networks. Cell Rep, 29(10):3331-3348.e7(2019) [pubmed] |
Keyword List
submitter keyword: metformin, diabetes, cancer, aging, AMPK, LKB1, liver, kinase, 14-3-3, SILAM, proteomics, PKD, MK2, STIM1, STIM2, calcium |
Contact List
John R. Yates III | |
---|---|
contact affiliation | The Scripps Research Institute |
contact email | jyates@scripps.edu |
lab head | |
Salvador Martínez de Bartolomé | |
contact affiliation | The Scripps Research Institute |
contact email | salvador@scripps.edu |
dataset submitter |
Full Dataset Link List
MassIVE dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000084429/ |