PXD015721

PXD015721 is an original dataset announced via ProteomeXchange.

Title	Brief exposure to heat differentially affects development of male gametophytes in cotton (Gossypium hirsutum)
Description	The development of viable gametes is especially susceptible to heat in all higher plants, including cotton (Gossypium hirsutum cv. Sicot 71), resulting in substantial reduction in lint quantity and quality at temperatures above 32 °C. Male reproductive cells are especially vulnerable to heat. This study demonstrates a relatively small impact of heat on leaves compared with a profound impact during early and late male gametophyte development. To investigate the mechanisms leading to heat sensitivity, the proteome of pollen was analyzed after two distinct phases of development (tetrads or binucleate microspores) had been exposed for 5 d to 36/25 °C (day/night) or 40/30 °C. The resulting mature pollen grains were collected for quantitative label-free shotgun proteomic analysis. A total of 868 proteins was quantified. Interestingly, Hsp70s were highly induced in response to extreme heat indicating key roles of this family to cope with heat stress.
HostingRepository	PRIDE
AnnounceDate	2020-10-19
AnnouncementXML	Submission_2020-10-19_01:26:27.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Farhad Masoomi-Aladizgeh
SpeciesList	scientific name: Gossypium hirsutum; NCBI TaxID: 3635;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-07 01:53:55	ID requested
⏵ 1	2020-10-19 01:26:28	announced

Masoomi-Aladizgeh F, Najeeb U, Hamzelou S, Pascovici D, Amirkhani A, Tan DKY, Mirzaei M, Haynes PA, Atwell BJ, Pollen development in cotton (Gossypium hirsutum) is highly sensitive to heat exposure during the tetrad stage. Plant Cell Environ, 44(7):2150-2166(2021) [pubmed]

submitter keyword: Cotton, Pollen development, Heat stress, LC-MSMS

Brian J. Atwell
contact affiliation	Department of Biological Sciences at Macquarie University
contact email	brian.atwell@mq.edu.au
lab head
Farhad Masoomi-Aladizgeh
contact affiliation	Macquarie University
contact email	farhad.masoomi-aladizgeh@hdr.mq.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/10/PXD015721

PRIDE project URI

• PRIDE
  1. PXD015721
     1. Label: PRIDE project
     2. Name: Brief exposure to heat differentially affects development of male gametophytes in cotton (Gossypium hirsutum)