PXD015717

PXD015717 is an original dataset announced via ProteomeXchange.

Title	Glycolytic profile shift and antioxidant triggering in symbiont-free and H2O2-resistant Strigomonas culicis
Description	During their life cycle, trypanosomatids are exposed to stress conditions and adapt their energy and antioxidant metabolism to colonize their hosts. Strigomonas culicis is a monoxenous protist found in invertebrates with an endosymbiotic bacterium that completes essential biosynthetic pathways for the trypanosomatid. Our research group previously generated a wild-type H2O2-resistant (WTR) strain that showed improved mitochondrial metabolism and antioxidant defenses, which led to higher rates of Aedes aegypti infection. Here, we assess the biological contribution of the S. culicis endosymbiont and reactive oxygen species (ROS) resistance to oxidative and energy metabolism processes. Using high-throughput proteomics, several proteins involved in glycolysis and gluconeogenesis, the pentose phosphate pathway and glutathione metabolism were identified. The results suggest that ROS resistance decreases glucose consumption and indicate that the metabolic products from gluconeogenesis are key to supplying the protist with high-energy and reducing intermediates. Our hypothesis was confirmed by biochemical assays showing opposite profiles for glucose uptake and hexokinase and pyruvate kinase activity levels in the WTR and aposymbiotic strains, while the enzyme glucose-6P 1-dehydrogenase was more active in both strains. Regarding the antioxidant system, ascorbate peroxidase has an important role in H2O2 resistance and may be responsible for the high infection rates previously described for A. aegypti. In conclusion, our data indicate that the energy-related and antioxidant metabolic processes of S. culicis are modulated in response to oxidative stress conditions, providing new perspectives on the biology of the trypanosomatid-insect interaction as well as on the possible impact of resistant parasites in accidental human infection.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:05:41.376.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Richard Hemmi Valente
SpeciesList	scientific name: Strigomonas culicis; NCBI TaxID: 28005;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2019-10-07 01:52:58	ID requested
1	2019-11-27 02:11:53	announced
⏵ 2	2024-10-22 04:05:49	announced	2024-10-22: Updated project metadata.

10.1016/j.freeradbiomed.2019.11.025;

Bomba, ç, a ACS, Brunoro GVF, Dias-Lopes G, Ennes-Vidal V, Carvalho PC, Perales J, d'Avila-Levy CM, Valente RH, Menna-Barreto RFS, -resistant Strigomonas culicis. Free Radic Biol Med, 146():392-401(2020) [pubmed]

submitter keyword: energetic metabolism, ascorbate peroxidase, pentose phosphate pathway, antioxidant system, oxidative stress

Richard Hemmi Valente
contact affiliation	Laboratory of Toxinology, FIOCRUZ, Brazil
contact email	richardhemmi@gmail.com
lab head
Richard Hemmi Valente
contact affiliation	Fundação Oswaldo Cruz
contact email	richardhemmi@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD015717

PRIDE project URI

• PRIDE
  1. PXD015717
     1. Label: PRIDE project
     2. Name: Glycolytic profile shift and antioxidant triggering in symbiont-free and H2O2-resistant Strigomonas culicis